From sbe at biochem.usyd.edu.au Sun Jun 1 22:02:49 1997 From: sbe at biochem.usyd.edu.au (Simon Easterbrook-Smith) Date: Mon Mar 7 07:29:59 2005 Subject: Affinity Constant References: <5CAC1671BB5@rna.bio.mq.edu.au> Message-ID: Hi Chris (1) Make Fab fragments from your Ab (I assume MAb, if not then you will not get a unique binding constant) and label the Fab with 125I. (2) Titrate labelled Fab into suspension of your ag-positive cells. (3) Measure free and bound Fab (from 125I radioactivity of cells and S/N) under equilibrium conditions (ie when [bound] is constant with time). (4) Plot [bound] vs [free], fit appropriate eqn to data (usually non-cooperative binding). (5) Best to correct for non-specific binding using labelled irrelevant (but isotype-matched) Fab. (6) Good luck. In article <5CAC1671BB5@rna.bio.mq.edu.au>, cweir@RNA.BIO.MQ.EDU.AU ("Chris Weir") wrote: || Hi all, || Could someone please be of assistance! || How do you determine the affinity constant of an antibody, when we || are unable to purify the antigen (as it is expressed on the surface || of the cell1)??? || || Thanks in advance || || Chris Weir || Macquarie University Simon -- _______________________________________________________________ Dr Simon B Easterbrook-Smith Voice: (+) 612 9351 3905 Department of Biochemistry FAX: (+) 612 9351 4726 University of Sydney Email: sbe@biochem.usyd.edu.au Sydney NSW 2006 AUSTRALIA _______________________________________________________________ Under U.S. Code, Title 47, Chapter 5, Subchapter II, ?227, all unsolicited commercial E-mail sent to my address from a U.S. account is subject to a fee of $500.00 U.S. By E-mailing me you accept these terms. cf. _______________________________________________________________ From EB15 at le.ac.uk Mon Jun 2 08:07:54 1997 From: EB15 at le.ac.uk (Dr E. Buxbaum) Date: Mon Mar 7 07:29:59 2005 Subject: Homemade Chemiluminescent Substrates? References: <338EFCA7.41C6@risotto.mit.edu> <338ECA5B.6690@uic.edu> Message-ID: <5mugja$1ds@falcon.le.ac.uk> Keld Sorensen wrote: >Say you use 10 ml of a 1:500 dilution of a primary antibody that cost >you $1,000/mg, then the antibody cost is $20 per blot. If you had to use >say a 1:250 dilution (weak affinity antibody) then the cost would be $40 >per blot!! This antibody solution would be reused many, many times. The costs per blot are, therefore, much lower. From ghermans at luc.ac.be Mon Jun 2 02:37:55 1997 From: ghermans at luc.ac.be (Guy Hermans) Date: Mon Mar 7 07:29:59 2005 Subject: Route of immunisation???? References: <338E908D.C9E@alpha2.curtin.edu.au> Message-ID: In article <338E908D.C9E@alpha2.curtin.edu.au>, Grace Ho wrote: > Hello everyone, > I am pretty confuse here about the route of immunisation!!! I am > currently working with a mouse model where I take my protein antigen in > Freund's adjuvant.... and immunised the victim via intraperitoneal route > .... in the hope of stimulating a good systemic response as well as good > T-cells reponse.... > However, I have been receiving advice from people (immunologists) > and found that ip. immunisation is not a good way to go about if I want > a good systemic/T-cells reponse..... They all recommended subcutaneous > injection or intradermal injection.... > Problem is I am so used to ip. route immunisation and my work have > been sort of based on ip. immunisation.... and things have not been > working very well for me... and on the other hand, if I change the route > of immunisation.. then my work is sort of down the drain!!!! > Does anyone have any insight to this!!!!????? Please!!!?? > Hello again Grace, there is a little insight available on this subject, yes. The route of immunisation is equally important in obtaining a 'good' immune respone as the choise of antigen & adjuvants. Apparently, the topological route of entry selects for a specific subset of antigen presenting cell. In other words; ip/freunds will preferentially use the intraperitoneal cavity macrophages as APC's, while the intrademal route will locate the antigen near the Langerhals cells there. These will take up the antigen and migrate to the draining lymph nodes, maturing to dendritic cells there. Both APC-derived cytokines and adhesion molecules on the APC seem to make a difference in the resulting response. The type of antigen presenting cell influences the response you'll get; therefore, route of entry will -indirectly- influence the response as well. Sorry to say , but your results obtained by ip immunisation will be hard to match with the ones obtained through id. However, you might make a point in your project by comparing the results of both methods. Best of luck, Guy -- Guy Hermans, PhD student Ms research Unit Immunology research group Dr. L. Willems-Institute Dept. of Physiology, LUC University Campus University Campus B-3590 Diepenbeek B-3590 Diepenbeek Belgium Belgium Voice ++32(0)11/26.92.07 Fax ++32(0)11/26.92.09 From mrc7 at cam.ac.uk Mon Jun 2 10:51:48 1997 From: mrc7 at cam.ac.uk (Mike Clark) Date: Mon Mar 7 07:29:59 2005 Subject: Affinity Constant References: <5CAC1671BB5@rna.bio.mq.edu.au> Message-ID: In article <5CAC1671BB5@rna.bio.mq.edu.au>, Chris Weir wrote: > > Hi all, > Could someone please be of assistance! > How do you determine the affinity constant of an antibody, when we > are unable to purify the antigen (as it is expressed on the surface > of the cell1)??? > > Thanks in advance > > Chris Weir > Macquarie University > Hi Chris, It really depends on how accurate you need to know the affinity constant? For most antibodies I usually refer to the avidity of binding ie the affinity constant for a whole antibody. The easiest way by far to get a very reasonable estimate of this constant which works for almost any detection system is to determine in a titration the concentration of antibody which gives 50% of maximal binding to antigen. If you then assume that the total input antibody is very nearly the same as free antibody (which is usually the case for antibody affinities) the kd is equal to this concentration. If you wish to express it as ka then just calculate the recipricol value. To get the single site affinity you could repeat the experiment with Fab fragments. Using computer curve fitting it is very easy to determine the 50% value with a high degree of accuracy and thus I rarely resort to transformations such as Scatchard Analysis. In fact the biggest error in this process is the determination of antibody concentration. Mike Clark, mrc7@cam.ac.uk http://www.path.cam.ac.uk/~mrc7/ -- o/ \\ // || ,_ o Dr. M.R. Clark, Division of Immunology <\__,\\ // __o || / /\, Cambridge University, Dept. Pathology "> || _`\<,_ // \\ \> | Tennis Court Rd., Cambridge CB2 1QP ` || (_)/ (_) // \\ \_ Tel.+44 1223 333705 Fax.+44 1223 333875 From cweir at RNA.BIO.MQ.EDU.AU Sun Jun 1 21:16:28 1997 From: cweir at RNA.BIO.MQ.EDU.AU (Chris Weir) Date: Mon Mar 7 07:29:59 2005 Subject: Affinity Constant Message-ID: <5CAC1671BB5@rna.bio.mq.edu.au> Hi all, Could someone please be of assistance! How do you determine the affinity constant of an antibody, when we are unable to purify the antigen (as it is expressed on the surface of the cell1)??? Thanks in advance Chris Weir Macquarie University From zjtangb at PUB.ZHANJIANG.GD.CN Mon Jun 2 08:48:43 1997 From: zjtangb at PUB.ZHANJIANG.GD.CN (zjtangb) Date: Mon Mar 7 07:29:59 2005 Subject: job-want Message-ID: <3393A77D.40CB@pub.zhanjiang.gd.cn> Dear Professor: My name is Tang Bin. I'm an associate professor of Immunology at Guangdong Medical College in Zhanjiang. I am writing to you to explore the possibility of spending one to two years working in your laboratory as a visiting scholar. Enclosed please find a complete CV of my education, training, researching , teaching experience, publications and references . As you can see from my CV, I have had experience in clinical immunology and immunopharmacology. I am now doing research in the role of cytokines(IL-4.6.8) & their receptors in chronic liver dieases, the molecular mechanism of several cytokines and adhesive molecules inducing glomerular mesangial cell apoptosis, the pathway of IFN-( inducing blood vessel endothelium cell growth and proliferation, etc. And also, doing research in immunological regulation of two kinds of BRMs(extracted from herbage). In the wake of these research, I am more interested in the following problems: 1) The clinical cytokines net. 2) The mechanism of cytokine and adhesive molecule as well as their mAbs in transducing singnals to lymphocytes or other cells. 3) The possibility of treating some diseases via regulating cytokine or adhesive molecule gene transcription and expression by biological response modifiers. I am eager to do something in these fields under your guidance. I am confident that I can do it better if you offer me this opportunity. I am confident of my ability to make a contribution, however small, to your ongoing research projects. My College has granted me an extended leave of absence, which enables me to obtain research experience and interaction abroad.I wish I could find words to express the importance of an appointment as a visiting scholar, for I know how I could learn, and how much China could gain from my working under your guidance. Please tell me whether or not you are in a position to offer me this opportunity. If convenient, please comment also on the possibility of my receiving any financial support. I hope all the above will give you a better idea of my background, and bring me a good luck. If you need any other additional information, please do not hesitate to contact me. Thank you for your consideration. I look forward to hearing from you soon. Yours sincerely, Dr Tang Bin Department of Microbiology & Immunology Guangdong Medical College Zhanjiang(524023), Guangdong PRC. E-mail: zjtangb@pub.zhanjiang.gd.cn Enclosure: Curriculum Vitae CURRICULUM VITAE Ⅰ. GENERAL INFORMATION Name: Tang Bin Description: 33 years old/male/married/178 cm/85 Kg Address: Department of Microbiology & Immunology, Guangdong Medical College, Zhanjiang(524023), Guandong, PRC Telephone: 86-0759-2281544-3035(Office) 86-0759-2234784-6003(Home) E-mail: zjtangb@pub.zhanjiang.gd.cn Fax: 86-0759-2284104 Citizen: PRC Date of birth: May 3, 1964 Place of birth: Guichi City, Anhui Province, PRC Health: excellent Ⅱ. EDUCATION 1989-1992: Wannan Medical College (Wuhu city, Anhui province, PRC) Department of Microbiology & Immunology, Postgraduate student and then got M.D. Major Courses: Immunology, Immunopharmacology, Pharmacology, Medical Microbiology, Cellular Genetics, Molecular Biology, Nuclear Medicine, Computer Science, Biochemistry, Medical Statistics, English, etc. 1979-1982: Chaohu Medical School, Anhui, PRC. Majoring in program of Medical Labrotary Technique. 1975-1979: Yinhui Middle School, Anhui, PRC Ⅲ. TRAINING 1. 17 April, 1995-27 August, 1995 Training program for cytokines research technique, Department of Immunology, Beijing Medical University, Beijing 2. 1 May, 1994-30 july, 1994 Training program for clinical immunology research technique, Clinical Immunology Laboratory of Changhai Hospital, Second Military Medical University, Shanghai Ⅳ. RESEARCHING & TEACHING EXPERIENCE 1992-present: Department of Microbiology & Immunology at Guangdong Medical College Current courses: Molecular Immunology (for graduate students), Medical Microbiology & Immunology(for undergraduates), Clinical Immunology & Immunologic test (and also for undergraduates ) Current reasearch: Clinical Immunology and Immunopharmacology 1989-1992: Department of Microbiology & Immunology at Wannan Medical College Research area: Immunopharmacology 1982-1989: Clinical Immunology Laboratory of Anqing Psychiatric Hospital, Anhui, PRC Ⅴ. PUBLICATIONS 1. Effect of stress on mice's immune function and hypothalamic-hypophyseal-thyroid axis. Shanghai Journal of Immunology. 1993; 13 (6): 340-341 2. The effect of TRH & Buzhong Yiqi Tang on RBC immunologic function recovery of amputation mice. Pharmacology and Clinics of Chinese Materia Medica. 1993; 9(4): 6-8 3. Effect of Buzhong Yiqi Tang on the recovery of red-cell immune adhesive function in amputated mouse. Journal of Guangdong Medical College. 1993; 11(4): 195-197 4. Advances in the immuno-pharmacological study of Astragalus mongolius. Journal of Guangdong Medical College. 1993; 11(3): 173-176 5. Advances in the study of anti-sperm immuno-sterility. Journal of Wannan Medical College. 1993; 12(3): 238-240 6. The effect of mixture of Astragalus mongolius and pig Ig on immunological function of mice. Journal of Wannan Medical College. 1993; 12(1): 5-7 7. Effect of TRH and Buzhong Yiqi Tang on NK activity and endocrine in stress mice. Chinese Journal of Integrated Traditional and Western Medicine. 1994; 14(2): 104-105 8. Effect of thyrotropin releasing hormone (TRH) on RBC immunologic function of mouse. Chinese Journal of Experimental & Clinical Immunology. 1994; 6(2): 4-6 9. Effect of thyrotropin releasing hormone on immune function in stressed mice. Chinese Journal of Pharmacology and Toxicology. 1994; 8(3): 233-234 10.Changes of red blood cell immune function in several kinds of nervous system infections diseases. Journal of Applied Clinical Pediatrics. 1994; 9(6):323-325 11.Advances in the experimental study of decoction Buzhong Yiqi. Journal of Guangdong Medical College. 1994; 12(4): 324-327 12.Investigation of red blood cell immune function in newborn common diseases. Journal of Proper Technique for Diagnosis and Therapy. 1994; 12(4): 4-5 13.ＴＲＨと补中益气汤の合用によるストレスマウスNK细胞の活性及び内分泌に 与える影响. The Journal of Chinese Traditional and Western Medicine(Japanese). 1995; 6(1): 93-95 14.Advances in the immunopharmacology study of Buzhong Yiqi Tang. Chinese Traditional Patent Medicine. 1995; 17(1): 42-43 15.Clinical significance of serum sIL-2R level in patients with cirrhosis. Chinese Journal of Clinical Hepatology. 1995; 11 (suppl): 16-17 16.Relationship between serum sIL-2R level and primary hepatocellular carcinoma. Shanghai Journal of Immunology. 1995; 15(2): 105-106 17.Erythrocytic immune function of idiopathic thrombocytopenic purpura. Chinese Journal of Hematology. 1995; 16(3): 130-131 18.Serum sIL-2R level in aged patients with primary hepatocellular carcinoma. Chinese Journal of Clinical Hepatology. 1995; 11(suppl):64-65 19.Clinical significance of Serum sIL-2R level variance in patients with heart failure. Chinese Journal of Critical Care Medicine. 1995; 15 (3): 4-6 20.Serum sIL-2R level in patients with digestive tract malignant tumor. Labeled Immunoaassays and Clinical Medicine. 1995; 2(2): 118-119 21.Experimental study on effect of Buzhong Yiqi Tang on red-cell immune function & hypothalamic-hypophyseal-thyroid axis in stress mice. Chinese Journal of Immunology. 1995; 11(Suppl): 300-302 22.Relationship between RBC-SOD, LPO and RBC immune function in patients with heart & brain vessel diseases. Chinese Journal of Immunology. 1995; 11(Suppl): 337-338 23.Study of relationship between sIL-2R and red-cell immune function in aged patients with gastric diseases. Chinese Journal of Gerontology. 1995; 15(special, second): 2-4 24. Clinical significance of serum sIL-2R variety in patients with heart & brain vessel diseases. Chinese Journal of Practical Medicine. 1995; 15(8): 474-475 25.Way to training experimental ability of undergraduates. Journal of Guangdong Medical College. 1995;13(4):369-370 26.Study of relationship between sIL-2R and red-cell immune function in patients with nasopharyngeal carcinoma. Journal of Clinal Immunology. 1995; 3(5):20-21 27.Study of relationship between sIL-2R and red-cell immune function in patients with kidney diseases. Shanghai Journal of Immunology. 1996; 16(1): 51 28.Role of TNF-α in heart & brain vessel diseases developing. Chinese Journal of Gerontology. 1995; 16(1): 4-5 29. Regulatory effect of Lingqi Anshen liquor on erythrocytic immune function and antioxidation in immunosuppressed mice. Chinese Journal of Integrated Traditional and Western Medicine.1996;16(3) :167-169 30.Role of sIL-2R in aged chronic bronchopneumonia. Guangdong Medical Journal. 1996; 17(4):240-241 31.Distinctive diagnostic value of testing sIL-2R in patients with tuberculosis or cancerous thoracal accumulated liquid. Journal of Practical Pulmonology. 1996; 3(1): 30-32 32.Clinical significance of erythrocytic immune adhensive function test in childhood's common diseases. Journal of Guangdong Medical College. 1996; 14(2): 127-128 33.Preliminary investigation of the erythrocytic immune function in children with malnutrition . Journal of Guangdong Medical College. 1996;14(3):236 34.A preliminary study on the relationship between serum interleukin-6 and acute cerebral infarction. Journal of Guangdong Medical College. 1996; 14(3): 216-217 35.Relationship between Serum sIL-2R level and red-cell immune fuction in patients with digestive ulcer. Shanghai Journal of Immunology. 1996; 16(3): inside front cover 36.Serum soluble IL-2 receptor level and RBC immune function in patients with chronic gastritis. Chinese Journal of New Gastroenterology. 1996; 4(9): 509-510 37.Study of RBC immune fuction in patients with bronchopneumonia.Chinese Clinical Medical. 1996; 1: 353-354 38.Pharmacological study on Lingqi Anshen liquor. Chinese Traditional Patent Medicine. 1996; 18(11):33-35 39.Role of IL-8 in chronic renal failure. Chinese Journal of Kidney. 1996; 12(6): 268-270 40.Serum level of IL-8 in systemic lupus erythematosus.Chinese Journal of Dermatology.1997;30(1):31 41.Role of TNF-α, IL-6 and IL-8 in primary hepatic carcinoma and cirrhosis. (has been accepted by Journal of Guangdong Medical College) 42.Study on the serum TNF-(, IL-6 & IL-8 levels before and after operation in patients with digestive tract tumor. (has been accepted by Shanghai Journal of Immunology) 43.Role of IL-6, IL-8 & C-GSF in the children's idiopathic thrombocytopenia purpura. (has been accepted by Chinese Journal of Hematology) Ⅵ. REFERENCES Chen Qun, Professor of Microbiology & Immunology, Chairman of the Department of Microbiology & Immunology, Guangdong Medical College, Zhanjiang(524023),Guangdong, PRC Wu Minyu, Professor of Immunology, my tutor, Chairman of the Department of Microbiology & Immunology, Wannan Medical College, Wuhu(241001), Anhui, PRC From amcbride at bilbo.bio.purdue.edu Mon Jun 2 14:01:52 1997 From: amcbride at bilbo.bio.purdue.edu (Ali McBride) Date: Mon Mar 7 07:29:59 2005 Subject: Homemade Chemiluminescent Protocols? References: <338EFCA7.41C6@risotto.mit.edu> Message-ID: <339318A0.270E@bilbo.bio.purdue.edu> David Aldridge wrote: > > In article <338EFCA7.41C6@risotto.mit.edu>, Thomas Cameron > wrote: > > > Company kits end up costing $3-5 per western blot which is 90% of the > > cost of the whole procedure. > > > > People must have some good homemade HRP (and AlkPhos) Chemiluminescent > > Detection protocols or references? > > > > Could you send me one or point me to it? Thanks. > > *Luminol 4mg/ml in DMSO 1ml > *p-iodophenol 1mg/ml in DMSO 1ml > 1M Tris.HCL pH 7.5 0.6ml > H2O2 (30%) 5 microL > H2O 7.5ml > > *Store at -20 > Mix this stuff up fresh a few minutes before use. > Works for me! Where can I buy the luminol and the p-iodophenol from Thanks in advance Ali From jrbagley at fas.harvard.edu Mon Jun 2 13:32:08 1997 From: jrbagley at fas.harvard.edu (Jessamyn Bagley) Date: Mon Mar 7 07:29:59 2005 Subject: IL-4 antibody Message-ID: <5mv3j8$eqk$1@news.fas.harvard.edu> I'm looking for a source of non-neutralizing anti-murine/human IL4. I need quite a bit of it, so I'd really like to get a hybridoma and purify the antibody myself. Any suggestions would be greatly appreciated! -- Jessamyn Bagley jrbagley@husc.harvard.edu From ecastro at physci.ucla.edu Mon Jun 2 11:20:19 1997 From: ecastro at physci.ucla.edu (Edmundo Castro) Date: Mon Mar 7 07:29:59 2005 Subject: Homemade Chemiluminescent Substrates? References: <338EFCA7.41C6@risotto.mit.edu> <338ECA5B.6690@uic.edu> Message-ID: <3392F2C3.7E7C@physci.ucla.edu> I have reused primary Ab >15 times with good results. Save $ =) EC From ahfell at netmatters.co.uk Mon Jun 2 11:38:10 1997 From: ahfell at netmatters.co.uk (Andrew Fell) Date: Mon Mar 7 07:29:59 2005 Subject: Route of immunisation???? References: <338E908D.C9E@alpha2.curtin.edu.au> Message-ID: <3392F6F2.5988@netmatters.co.uk> Guy Hermans wrote: > > Apparently, the topological route of entry selects for a specific subset > of antigen presenting cell. In other words; ip/freunds will preferentially > use the intraperitoneal cavity macrophages as APC's, while the intrademal > route will locate the antigen near the Langerhals cells there. These will > take up the antigen and migrate to the draining lymph nodes, maturing to > dendritic cells there. Both APC-derived cytokines and adhesion molecules > on the APC seem to make a difference in the resulting response. > > The type of antigen presenting cell influences the response you'll get; > therefore, route of entry will -indirectly- influence the response as > well. > > Sorry to say , but your results obtained by ip immunisation will be hard > to match with the ones obtained through id. However, you might make a > point in your project by comparing the results of both methods. > Grace, I should do some Medline searching on this. I think there is literature out there on route of immunisation and this is one of those subjects where every Ph.D. student re-invents the wheel. BTW, an excellent way to use Medline is the NCBI Pubmed search engine at http://www.ncbi.nlm.nih.gov/PubMed Personally, I think material injected into the PEC drains into the blood quickly and then gets pretty much everywhere. However, I guess the Freunds might keep it in the PEC as a big oily blob. Andy Fell From liawas at pc.jaring.my Mon Jun 2 17:25:54 1997 From: liawas at pc.jaring.my (Lea Johnsiul) Date: Mon Mar 7 07:29:59 2005 Subject: OUTBREAK OF ACUTE VIRUS INFECTION IN SARAWAK, MALAYSIA. Message-ID: <33934871.4A65@pc.jaring.my> An outbreak of an acute virus infection was detected in Sarawak, Malaysia beginning from 14 April 1997. Up till today (2 June 1997), 15 children have died from this disease. Their ages ranged from 5 months to 3.5 years; nine were males and six were females. All the cases came from different localities, but six were from within the same district. All cases were very ill when admitted to hospital and died within 24 hours of admission. All presented with similar clinical features which were as follows:- Clinical features: - fever of 2-3 days duration - involvement of the nervouse system which manifested as fits and paralysis of one side of the upper limbs - poor systemic perfusion - end stage : cardiogenic shock due to myocarditis. Echocardioram shows poor contractability of left ventricle Other significant findings, Chest x-ray - pulmonary oedema Cerebro-spinal fluid -suggestive of aseptic meningitis the following have been excluded serologicay : Japanese encephalitis Dengue Yellow fever Virus culture: Serum and cerebro-spinal fluid cultured in: 1. baby hamster kidney cells medium MEM with 1% foetal bovine serum 2. pig kidney cells (PS) medium Leibovitz 15 with 1% foetal bovine serum 3. mosquito cells C6/36 medium Leibovitz 15 with 1% foetal bovine serum Cytopathic effect (CPE) noted in medium (3) for cerebrospinal fluid from one patient Throat and rectal swabs will be cultured in vero-primate cell line today (2/5/1997) As we have yet to come to a conclusive diagnosis of this condition, we would bevery grateful if anyone could provide some information or suggestion as to what organism we are dealing with. This is essential for control purposes. Please contact : Sarawak Health Department Email : JKNS2@po.jaring.my Tel: 60-82-256566 Fax: 60-82-424959 From ronlab at UIC.EDU Mon Jun 2 15:25:13 1997 From: ronlab at UIC.EDU (ronlab) Date: Mon Mar 7 07:29:59 2005 Subject: Q: protein A instead of a secondary Ab Message-ID: <33932CC9.3DA@uic.edu> Hi! I would like to know if there are any limitations on using protein A or protein G instead of a secondary Ab for immunostaining and FACS. Does anyone has relevant experience (positive or negative)? Are there any published protocols for these applications? Your suggestions and comments will be greatly appreciated. Eugene Kandel. U09577@uic.edu From tore001 at pn.itnet.it Mon Jun 2 16:09:50 1997 From: tore001 at pn.itnet.it (AT) Date: Mon Mar 7 07:29:59 2005 Subject: Helicobacter sito ITALIANO Message-ID: <5mvcs9$seq@dns2.IT.net> Helicobacter - sito ITALIANO For ITALIAN people: the most complete site on Helicobacter pylori is at: VI SFIDO A TROVARE UN SITO CON MAGGIORI INFORMAZIONI ------------------------------------------- dr Alberto Torelli (1997) tel/sgr/fax 019-506054 Alberto.Torelli@pn.itnet.it ------------------------------------------- From dmike at ix.netcom.com Mon Jun 2 22:15:43 1997 From: dmike at ix.netcom.com (David J. Mike) Date: Mon Mar 7 07:30:00 2005 Subject: Need help! for parisitic illness in Thailand Message-ID: <5n01sf$4q0@dfw-ixnews6.ix.netcom.com> I am looking for help for a young missionary in Thailand as follows: TO MY "URGENT PRAYER WARRIOR" NETWORK: While at the 24 hour prayer desk at the U.S. Center for World Mission today we received this URGENT PRAYER e-mail for David and Michelle Allen, missionaries to Thailand. I think they are with MAF. Their e-mail address snadvm@flash.net.>817-451-0257 (I assume the numbers are a phone number: THE NEED FOR PRAYER David has an invasion of his body by unknown pasasites and apparently only has 2 months to live if God does not intervene. He is a young missionary to Thailand with a 4 month old daughter, Brianna. He comes from a long line missionary family. Please lift David and his family before the throne of grace. Below is quoted from David: "My condition is quite serious now. The body is beginning to break down because I have no more fat or nutrient reserves. My diet consists mostly of vegebroth, gatorade, and saltines. I tried homemade bread a few weeks ago and ended up in the emergency room. I am in constant pain and have to take pain killers regularly. In the last 3 days there have been sharp pains in both kidneys. So far, eight Drs. have not been able to diagnose the parasites. So they are now running tests to see if my kidneys are infected.... They have found two foreign agents, but no one can ID them. One of the effects is to prevent my GI tract from absorbing nutrition. CDC in Atlanta is 3-6 months behind, so they can not help in time. The Dr's. are trying everyone else- they have pictures of the parasite in circulation, trying to get it identified. I am not doing well. I feel like I am in a very dark valley right now. I have been praying for so long for help with no response, that I have become discouraged in prayer. This is a first for me in my life. My prayers are now very elemental:"Father save me!" But the pain continues each day, and I continue to lose weight (lost over 30# to date). Please pray not only for my body but for my spirit. I have not known fear like this before. I don't want to be fearful, and I don't need to be fearful because I am confident in my salvation. I think my fear is related more to the thought of not being with my wife and new baby. This was the happiest time in my life before I became sick." Do you know anyone who can offer David help? Perhaps you can forward this e-mail to someone who is knowlegeable in the area of parasitic disease. I would appreciate any help you can offer. Thank you David J. Mike From Keith.Cienkus at add.ssw.abbott.com Tue Jun 3 13:47:19 1997 From: Keith.Cienkus at add.ssw.abbott.com (Cienkus,Keith) Date: Mon Mar 7 07:30:00 2005 Subject: Veterinary Immunology Message-ID: <0017000001005968000002L082*@MHS> Does anyone know of information relating to complement testing of goats and/or know of any companies that perform this type of testing? My e-mail address is cienkkr.add@notes.abbott.com From KeldS at uic.edu Tue Jun 3 03:45:43 1997 From: KeldS at uic.edu (Keld Sorensen) Date: Mon Mar 7 07:30:00 2005 Subject: Q: protein A instead of a secondary Ab References: <33932CC9.3DA@uic.edu> Message-ID: <3393D9B6.18C7@uic.edu> Remember protein A does not like all subclasses, so probably protein G would be preferred. Protien A and Protein G will pick up Fc receptors on cell surfaces - so will intact secondary Ab (most people would use Fab or Fab2 fragments). Keld. From arruda at SVN.COM.BR Tue Jun 3 16:30:00 1997 From: arruda at SVN.COM.BR (sergio arruda) Date: Mon Mar 7 07:30:00 2005 Subject: NK and CD1 Message-ID: <3394C9C5.23E@svn.com.br> Netters, Anyone up there knows about papers or reviews about human NK and CD1 molecules. Thanks for help me arruda@svn.com.br From PERLA at MAILBOX.HSCSYR.EDU Tue Jun 3 17:48:57 1997 From: PERLA at MAILBOX.HSCSYR.EDU (Andras Perl) Date: Mon Mar 7 07:30:00 2005 Subject: POSTDOCTORAL POSITIONS Message-ID: RESEARCH POSITIONS APPLICATIONS ARE INVITED FOR RESEARCH POSITIONS TO STUDY 1) THE STRUCTURE AND EXPRESSION OF THE HUMAN TRANSALDOLASE GENE AND AN ASSOCIATED HUMAN REPETITIVE ELEMENT (J. BIOL. CHEM. 269: 2847-2851, 1994) 2) OLIGODENDROCYTE-SPECIFIC EXPRESSION AND AUTOANTIGENICITY OF HUMAN TRANSALDOLASE IN PATIENTS WITH MULTIPLE SCLEROSIS (J.EXP.MED. 180:1649-1663, 1994; J. Clin. Invest. 99:123801250, 1997), AND 3) ROLE OF TRANSALDOLASE IN APOPTOTIC SIGNALING (J. BIOL. CHEM, 271:32994-33001, 1996) WITH A SPECIAL EMPHASIS ON OLIGODENDROCYTES AND LYMPHOID CELLS. EXPERIENCE IN MOLECULAR BIOLOGY, IMMUNOLOGY, AND/OR SIGNALING MECHANISMS IS REQUIRED. APPLICANTS SHOULD SEND A LETTER EXPRESSING THEIR INTEREST AND QUALIFICATIONS, A CURRICULUM VITAE, AND THE NAMES, ADDRESSES, AND TELEPHONE NUMBERS OF THREE REFERENCES TO: DR. ANDRAS PERL, ASSOCIATE PROFESSOR OF MEDICINE AND MICROBIOLOGY AND IMMUNOLOGY, STATE UNIVERSITY OF NEW YORK HEALTH SCIENCE CENTER, SYRACUSE, NY 13210, USA From EB15 at le.ac.uk Tue Jun 3 07:26:37 1997 From: EB15 at le.ac.uk (Dr E. Buxbaum) Date: Mon Mar 7 07:30:00 2005 Subject: Homemade Chemiluminescent Protocols? References: <338EFCA7.41C6@risotto.mit.edu> <339318A0.270E@bilbo.bio.purdue.edu> Message-ID: <5n12ht$prs@falcon.le.ac.uk> Ali McBride wrote: >Where can I buy the luminol and the p-iodophenol from >Thanks in advance >Ali 4-Iodophenol is available from Aldrich, Luminol from Fluka. Warning: Do not buy Luminol from Sigma, we had a complete failure with that stuff once. From hk-miami at ix.netcom.com Tue Jun 3 20:02:30 1997 From: hk-miami at ix.netcom.com (HK) Date: Mon Mar 7 07:30:00 2005 Subject: Homemade Chemiluminescent Protocols? References: <338EFCA7.41C6@risotto.mit.edu> <339318A0.270E@bilbo.bio.purdue.edu> <5n12ht$prs@falcon.le.ac.uk> Message-ID: <5n2er6$n0m@dfw-ixnews8.ix.netcom.com> x-no-archive: yes In <5n12ht$prs@falcon.le.ac.uk> "Dr E. Buxbaum" writes: >Ali McBride wrote: >>Where can I buy the luminol and the p-iodophenol from >>Thanks in advance >>Ali >------------------------------------- >4-Iodophenol is available from Aldrich, Luminol from Fluka. Warning: >Do not buy Luminol from Sigma, we had a complete failure with that stuff once. ---------------------------------------------- Maybe it wasn't the luminol...Maybe you mixed or measured something incorrectly, since you said that you only tried it once. I buy Luminol from Sigma...it works just fine. In a side-by-side test of my solutions and the ones in the Amersham ECL kit, my solutions work equally as well. Helene From CURRYBROWN at aol.com Tue Jun 3 22:30:07 1997 From: CURRYBROWN at aol.com (CURRYBROWN@aol.com) Date: Mon Mar 7 07:30:00 2005 Subject: goats and complement Message-ID: <970603232912_677702572@emout09.mail.aol.com> Does anyone have experience with measuring complement in goat sera? Also are there companies and/or universities that have a working complement assay for goats? Rick From maxmass at iol.it Wed Jun 4 15:14:52 1997 From: maxmass at iol.it (Massimo Massaia) Date: Mon Mar 7 07:30:00 2005 Subject: TCR-transfected mature T cells Message-ID: <3395CE9B.18ED@iol.it> Does anybody know what happens if I try to transfect a CDR3-specific sequence in a mature T cells, already expressing a self-rearranged TCR? Does it make sense? Massimo Massaia maxmass@iol.it From synt at IBCH.SIOBC.RAS.RU Wed Jun 4 13:50:42 1997 From: synt at IBCH.SIOBC.RAS.RU (Trakhanova Marina) Date: Mon Mar 7 07:30:00 2005 Subject: Announce: WWW Syntesome GmbH for glycoscientists Message-ID: <3395B9AC.2C2B@ibch.siobc.ras.ru> Dear Colleagues! We have the pleasure to inform you that Syntesome has now a home page (http://www.syntesome.ru/) where you can find information about the following: Reagents for glycobiology Application of neoglycoconjugates in academic and industrial laboratories Custom synthesis of saccharides The company's possibilities in the field of development of assay systems Fields of potential cooperation of Syntesome with pharmaceutical companies in glycotechnology Individual section of this home page are dedicated to: Assay Selectins Glycosyltransferases Generation of antibodies Cytochemistry/Histochemistry Hematology/Blood typing Trasplantation/Xenotransplantation Oncology: markers, targeting, vaccines Virology Neurobiology Study of lectins-receptors and search of their antagonists ------------------------------------------------------------------------------------------------------------------ Syntesome, Gesellschaft fur medizinische Biochemie mbH was founded in 1993 and is active in Glycobiology and Glycotechnology, important and growing fields in life sciences. Our catalogue includes about 200 products, mainly glycoconjugates, which are the result of many years of experience, which our scientific staff has acquired in the academic world. It is the company's ambitious endeavour to provide top performance and to occupy and maintain a leading position in this field. Our commitment is not only to find high level solutions to problems by developing high quality carbohydrate-based biochemicals, but to offer our customers in the scientific world a collection of new and unique products, giving them the possibility to design and perform experiments which were impossible previously. The range of products and services we offer is mainly orientated on the study of the functions of carbohydrates as well as the search and study of carbohydrate-binding proteins in biological processes, and on the discovery of new carbohydrate-mediated interactions. Due to the great flexibility and reproducibility of our synthetic technology, we are in the position to rationaly design and produce new glycoconjugates perfectly suited for each particular applications. We already have a great experience in collaborative research with pharmaceutical companies and academic institutions. At any time we are ready to help our customers in the design and production of a new glycoconjugates, in the development of test-systems for diagnostics and pharmaceutical screening, or in the study of the fine specificity of carbohydrate-binding proteins. --------------------------------------------------------------------- For FREE catalogue mail us synt@ibch.siobc.ras.ru Trakhanova Marina synt@ibch.siobc.ras.ru Syntesome GmbH http://www.syntesome.ru/ From Schmidtl at PPRZ02.HRZ.UNI-MARBURG.DE Wed Jun 4 15:49:27 1997 From: Schmidtl at PPRZ02.HRZ.UNI-MARBURG.DE (Fabian Schmidt) Date: Mon Mar 7 07:30:00 2005 Subject: Surface antigens in fetal mouse tissue Message-ID: <3396215B.3C6@stud-mailer.uni-marburg.de> To whoever can help me : I am looking for a up-to-date overview of the time of appearence and expression of surface antigens in fetal mice. Specially of those involved in the thymic development and the development of paraganglia. I am thankful for every hint. Fabian Schmidt University of Marburg, Germany From yksuen at netvigator.com Wed Jun 4 10:19:54 1997 From: yksuen at netvigator.com (Suen Yick Keung) Date: Mon Mar 7 07:30:00 2005 Subject: Dissolve Zinc Chloride in Cell Culture Medium Message-ID: <01bc70f7$cfe11900$a5628bd0@default> I am going to test the possible inhibitory effect of zinc ions on apoptosis of murine macrophage cell line. It is well known that zinc can inhibit apoptosis in many cell types and I found there are many papers showing reduction of apoptosis of culture cells in the presence of zinc chloride. At the beginning of my experiment, I faced the most fundamental but important problem: the solubility of zinc chloride in neutral pH culture medium. Obviously, the zinc chloride cannot dissolve in the medium when the pH is near neutral (around pH 7.2). No paper (as far as I have obtained) mentioned this (may be it is too simple). And I was unable to find the solution from any chemistry books. Do anyone know the answer ? Please Email to me. Thanks !! Y.K.Suen Email : yksuen@netvigator.com From mandywj at mail.utexas.edu Wed Jun 4 09:55:39 1997 From: mandywj at mail.utexas.edu (WILLIAM J MANDY) Date: Mon Mar 7 07:30:00 2005 Subject: goats and complement References: <970603232912_677702572@emout09.mail.aol.com> Message-ID: <5n3vlb$jbv$1@geraldo.cc.utexas.edu> In article <970603232912_677702572@emout09.mail.aol.com>, CURRYBROWN@aol.com says: Rick: Apparently goats and sheep have problems with unusually high levels of anti-complimentary activity. You may be better off with a different assay if possible. What are you testing? Bill ( mandywj@mail.utexas.edu ) > >Does anyone have experience with measuring complement in goat sera? Also are >there companies and/or universities that have a working complement assay for >goats? > >Rick From EB15 at le.ac.uk Wed Jun 4 12:16:40 1997 From: EB15 at le.ac.uk (Dr E. Buxbaum) Date: Mon Mar 7 07:30:00 2005 Subject: Homemade Chemiluminescent Protocols? References: <338EFCA7.41C6@risotto.mit.edu> <339318A0.270E@bilbo.bio.purdue.edu> <5n12ht$prs@falcon.le.ac.uk> <5n2er6$n0m@dfw-ixnews8.ix.netcom.com> Message-ID: <5n47to$188@falcon.le.ac.uk> hk-miami@ix.netcom.com(HK) wrote: >Maybe it wasn't the luminol...Maybe you mixed or measured something >incorrectly, since you said that you only tried it once. >I buy Luminol from Sigma...it works just fine. In a side-by-side >test of my solutions and the ones in the Amersham ECL kit, my >solutions work equally as well. I would not say things like this if I hadn't done the ovious tests. In general I had quite a few problems with Sigma's chemicals over the years, for example SDS which was insoluble in water, inactive enzyme... Looks like a quality control problem to me. From o.h.brekke at bio.uio.no Wed Jun 4 08:57:05 1997 From: o.h.brekke at bio.uio.no (Ole Henrik Brekke) Date: Mon Mar 7 07:30:00 2005 Subject: Post Doc position Message-ID: <5n3s7h$2ie$1@ratatosk.uio.no> UNIVERSITY OF OSLO Postdoc position in Immunology/Cell biology (up to 3 yrs) EC-TMR Postdoc position for EC citizen and associated states. Participation in TMR Research Networks: "Intracellular mechanisms of antigen processing and presentation by the MHC Class I and Class II molecules" and "Sorting of endosomal and lysosomal proteins by molecular machineries" ERBFMRXCT960069 og ERBFMRXCT960058 (http://www.cordis.lu/tmr/home.html). Possible research visit to several collaborating laboratories. Salary: aprox. 3000 ECU/mth Only citizens of EC member state + Iceland and Israel. Further info and applic.: Dr. Oddmund Bakke, Dep. of Molecular Cell Biology, Box 1050, University of Oslo, N- 0316 Oslo. email: obakke@bio.uio.no. Phone +47 22855787 fax: +47 22854605 From o.h.brekke at bio.uio.no Wed Jun 4 08:51:24 1997 From: o.h.brekke at bio.uio.no (Ole Henrik Brekke) Date: Mon Mar 7 07:30:00 2005 Subject: Post Doc position in Norway Message-ID: <5n3rss$2hd$1@ratatosk.uio.no> UNIVERSITY OF OSLO Postdoc position in Immunology/Cell biology (up to 3 yrs) EC-TMR Postdoc position for EC citizen and associated states. Participation in TMR Research Networks: "Intracellular mechanisms of antigen processing and presentation by the MHC Class I and Class II molecules" and "Sorting of endosomal and lysosomal proteins by molecular machineries" ERBFMRXCT960069 og ERBFMRXCT960058 (http://www.cordis.lu/tmr/home.html). Possible research visit to several collaborating laboratories. Salary: aprox. 3000 ECU/mth Only citizens of EC member state + Iceland and Israel. Further info and applic.: Dr. Oddmund Bakke, Dep. of Molecular Cell Biology, Box 1050, University of Oslo, N- 0316 Oslo. email: obakke@bio.uio.no. Phone +47 22855787 fax: +47 22854605 From dseegert at aol.com Wed Jun 4 15:40:31 1997 From: dseegert at aol.com (Dirk Seegert) Date: Mon Mar 7 07:30:00 2005 Subject: Wanted: TNFa knock out macrophages Message-ID: <5n3k16$sb2$2@news.fhg.de> Hi there, I'm involved in studying regulatory mechanisms of TNFa expression. For this pupose I have made some TNF construct which I want now to transfect into macrophage cellines to see whether they are inducible by different stimuli. What I'm looking for are one or more human macrophage cellines (THP-1 or U937 prefered) which are not able to produce TNFa after stimulation with LPS or IFNg. Is anybody able to provide me with such cells. May be a collaboration is possible!? Looking forward for your answer Dirk Seegert, Ph.D. Fraunhofer Institute Dept. Immunobiology Nikolai-Fuchs-Str. 1 D-30625 Hannover Fax: +49 (511) 5350 155 Tel. +49 (511) 5350 255 From ayelod at post.tau.ac.il Wed Jun 4 04:43:49 1997 From: ayelod at post.tau.ac.il (oded singer) Date: Mon Mar 7 07:30:00 2005 Subject: X-gal assay for lymphocytes Message-ID: <5n3dcl$2l0@mserv1.dl.ac.uk> I am trying to express beta-gal in lymphocytes. I am using a plasmid that contains the beta-gal gene attached to CMV promotor and this plasmid was electroporated into a T-lymphocytes cell line (JJhan). This plasmid was expressed efficiently in non-lymphoid cell line. I could not detect any beta-gal activity in the T-lymphocytes using the X-gal staining method. Does any one know about any successful X-gal assay protocol for lymphocytes or any reason why this assay is not working in lymphocytes? I must say that we are using electroporation (gene pulser) routinely to transfect T-lymphocytes with plasmids and transfected plasmid DNA can be easily detected in southern blott. Thanks, Oded From pnavarro at inav.net Tue Jun 3 23:19:09 1997 From: pnavarro at inav.net (Pedro A Navarro) Date: Mon Mar 7 07:30:00 2005 Subject: RPA kits from Ambion Message-ID: <01bc709e$baca6020$e46b78c7@pnavarro.inav.net> Does anybody know if Ambion's "Direct Protect Kit" offers any real advantages over their RPA II kit, or any comparable RPA kit? I wonder if it works on all the tissues. My research involves working on blood, liver, and lymphoid organs. The idea of not having to isolate the RNA prior to the assay seems attractive, but I wonder if there is any downside to this. Thanks for your time! _ _____________________________________ _ / ) | Pedro A Navarro | ( \ _( (_ | The Univ.of Iowa, Micro Dept. | _) )_ (((\ \>|_/->_____________________________<-\_| Message-ID: <339578C8.5F2C@whoi.edu> David Aldridge wrote: > *Luminol 4mg/ml in DMSO 1ml > *p-iodophenol 1mg/ml in DMSO 1ml > 1M Tris.HCL pH 7.5 0.6ml > H2O2 (30%) 5 microL > H2O 7.5ml > > *Store at -20 > Mix this stuff up fresh a few minutes before use. > Works for me! I assume this is for HRP, does it work for AP, too? If not, does anybody have one for AP? Eli Hestermann -- Eli V. Hestermann ehestermann@whoi.edu Woods Hole Oceanographic Institution Massachusetts Institute of Technology "Vita brevis est, ars longa" - Seneca From knecht at uconnvm.uconn.edu Wed Jun 4 12:54:43 1997 From: knecht at uconnvm.uconn.edu (David Knecht) Date: Mon Mar 7 07:30:01 2005 Subject: Macrophage or neutrophil chemotaxis to fMLP Message-ID: <3395ABB4.13B@uconnvm.uconn.edu> We are trying to repeat some old neutrophil chemotaxis experiments with neutrophils and macrophages responding to fMLP. We have isolated mouse peritoneal cells before and after thioglycolate injection adn then simply looked for random motility stimulated by uniform fMLP in buffer or media. The cells come out very round and rarely attach to glass or plastic, spread or move before or after fMLP addition. Is there some trick we are missing here that gives activatable cells? Thanks, Dave From marann at bconnex.net Wed Jun 4 19:48:59 1997 From: marann at bconnex.net (Dr. Martin Nemec) Date: Mon Mar 7 07:30:01 2005 Subject: Seeking post-doc in immunology Message-ID: <01bc7149$155d0420$a90e05d1@martin.bconnex.net> I am looking for my first post-doctoral post. I received my Ph.D. in immunology from the University of Glasgow, Scotland in the summer of 1996. I am interested in all areas of immunology, especially the mechanisms involved in intracellular signaling. I have used a variety of methods for studies at the cell, as well as molecular level. Also, I was involved in development, characterization and application of monoclonal antibodies. I have extensive experience in computing, including hardware and software setup and programming. For my academic and employment history please see my resume at: http://www.bconnex.net/~marann/mcv.html. Sincerely, Martin Nemec. From syslan2 at net.disbumad.es Thu Jun 5 00:03:24 1997 From: syslan2 at net.disbumad.es (Javier Casas Ciria) Date: Mon Mar 7 07:30:01 2005 Subject: Chlamydia pneumoniae and atherosclerosis Message-ID: <3396489C.15ED@net.disbumad.es> READ THE FULL TEXT ARTICLE The Link Between Chlamydia pneumoniae and Atherosclerosis ------------------------------------------------------------- There is a growing body of evidence suggesting that coronary artery disease is caused by an infectious agent. The leading candidate is Chlamydia pneumoniae, based on seroepidemiologic and histopathologic studies. in this page: When the representative articles on this topic are analyzed surprising data and apparently contradictory can be found. A datum that Saikku et al. considered fundamental and carries to them to establish this association, is that the patients with acute myocardial infarction or with coronary hearth disease were presenting geometric average of IgG greater than control group (P. Saikku, 1998). However, in 1993, Kuo et al demonstrate the presence of C. pneumoniae through PCR and electron microscope in plaques of atheroma from coronary. Precisely in those that previously to their death were presenting low titles of IgG or absence (C.C. Kuo, 1993). This apparent discrepancy seems very difficult to conciliating through clinic experiment and perhaps alone could now to speculate as of indirect data to explain these publications. A possible explanation would be in the immune capacity of the body against to the systemic infection by C. pneumoniae. A good immune response to an infection by chlamydia can produce distortion of membranes of chlamydia, losing its typical morphology and in a way aberrant appearance with difficulty identificable to the optical microscope. In this case certainly it will appear in blood antibodies in microimmunofluorescence. If is not produced an adequate immune response chlamydia maintains its morphology and antibodies can be not detected in serum by microimmunofluorescence (R. Malinverni, 1996). On the need noted by Muhlestein of infectious animal models of appearance of atherosclerotic plaque by the infection by C. pneumoniae to consider a causal relationship of C. pneumoniae already it has been published by Fong et al. in Journal of Clinical Microbiology, January of 1997 (I.W. Fong, 1997). On the other hand if we take as certain the causal association of C. pneumoniae with the coronary pathology it should be of outlining the boarding of this problem. A first possibility would be the vaccine. But as occurs with the trachoma and C. trachomatis there can not to solve the problem, since in one moment of the evolution of trachoma, immune stimulation is prejudicial in the evolution of the process. Other possibility would be the antibiotic treatment. On this has been said that upon trying a great infected population resistances can be created to erythromycin or tetracyclines. This phenomenon can not prevent its therapeutical use in this process as either prevents it in trachoma. Just as in trachoma, in advanced and very chronic injuries the antibiotic treatment there can not to alter the evolution of the coronary disease caused by this germ. For this is necessary to make an early diagnosis of the chronicle infection by C. pneumoniae. Thus we should develop a exact diagnostics technique for the diagnosis of the chronicle infection by C. pneumoniae. Malinverni R. 1996. The role of cytokines in chlamydial infections. Curr Opin Infect Dis 9, 150-155. Kuo CC, Shor A, Campbell LA, Fukushi H, Patton DL, Grayston JT. 1993. Demonstration of Chlamydia pneumoniae in atherosclerotic lesions of coronary arteries. J. Infect. Dis. 167(4):841-9. Saikku p, leinonen m, mattila k, ekman mr, nieminen ms, makela ph, huttunen jk, valtonen v. 1988. Serological evidence of an association of a novel Chlamydia, TWAR, with chronic coronary heart disease and acute myocardial infarction. Lancet. 2(8618):983-6 Fong IW, Chiu B, Viira E, Fong MW, Jang D, Mahony J. 1997. Rabbit model for Chlamydia pneumoniae infection. J. Clin. Microbiol. 1997;35:48-52 From mcpart at nccn.net Thu Jun 5 13:03:17 1997 From: mcpart at nccn.net (Brian P. McPartland, O.D.) Date: Mon Mar 7 07:30:01 2005 Subject: Is there a way to deminish IL-2 excess, without disrupting T-cell and NK cell activity? From a novice immunologist. Message-ID: <5n6v15$mkt@nccn3.nccn.net> Any ideas? Perhaps via attempts to regulate CD 8+/CD 28 molecules? does this make any sense? Brian From lambu at biochem.iisc.ernet.in Thu Jun 5 11:58:05 1997 From: lambu at biochem.iisc.ernet.in (Ashok M S) Date: Mon Mar 7 07:30:01 2005 Subject: hemophagocytosis Message-ID: <5n6r6t$88o@mserv1.dl.ac.uk> hi, i would like to know what is hemophagocytosis. any reference about it or the protocol of it would be helpful. thankyou pl reply to george@biochem.iisc.ernet.in or by newsgroup. karthik dept of biochemistry indian institute of science bangalore india From ans033 at sysc.abdn.ac.uk Thu Jun 5 10:45:45 1997 From: ans033 at sysc.abdn.ac.uk (m.g.blaylock) Date: Mon Mar 7 07:30:01 2005 Subject: IL-4 positive CD3-ve cells Help? Message-ID: <5n6mv9$221@info.abdn.ac.uk> I have been doing some flow cytometry on T cells and i am trying to find the balance of T helper cell one and two subgroups by measuring the amount of il-4 and IFN GAMMA, i have been having trouble getting any IL-4 staining with a FITC anti human IL-4, on CD4 positive cells, so to test my antibody i did the test with a different antibody to IL-4 conjugated with PE, but as i only had a FITC CD3 antibody t wasn't specific for T helper cells. I found i had good staining for IL-4 both on CD3 positive and negative cells, and i am having trouble finding out what group of cells are CD3 negative and produce IL-4 on stimulation. I have gated on lymphocytes only on the flow cytometer so they are not anything else such as mast cells. i would be grateful for any suggestions, and would like to know if any-one else has had trouble getting IL-4 production from activated T cells from healthy volunteers. Anna From assist at soback.kornet.nm.kr Thu Jun 5 05:12:00 1997 From: assist at soback.kornet.nm.kr (Oh Hwa-gyun) Date: Mon Mar 7 07:30:01 2005 Subject: Looking for the source of CD5 and CD3 Message-ID: <01bc7198$c38b8680$17d07ea8@KORNET.kornet.nm.kr> i'm looking for the source of human CD5 and CD3 molecules. i have been anti-human CD5 monoclonal ab. with jurkat cell-line but it was not good immunogen. so, i have found the purified human CD5 and expressed it in the prokaryotic system. badly, Expressed human CD5 was not efficient for the immunogen. i need it , i must get it,,,, please, any comments would be so greatly appreciated ! Hawgyun Oh From sergey at guiness.ksisti.alma-ata.su Thu Jun 5 00:05:26 1997 From: sergey at guiness.ksisti.alma-ata.su (Sinenko Sergey Anatolievich) Date: Mon Mar 7 07:30:01 2005 Subject: New MAB to superantigene Message-ID: Dear audience, We have obtained monoclonal antibody to immobilized ( not free ) superantigen staphilococcal enterotoxin A (SEA). We are interested the research by MAb of action mechanism SEA as immunomodulator (action on NK cells) . We search for to the interested researchers therefore to a question, form of collaboration can be from the granting MAb up to joint Grants. We wait for the proposals. Thank you Sergey Sinenko A. Junior scientific employee 480012, Kazakstan, Almaty, Ajtkhozhin`s Institute of Molecular Biology and Biochemistry (IMBB), 86 Michurin St. Tel. 7(3272)347092, E-male: adm@bioch.academ.alma-ata.su From vblasch at gwdg.de Wed Jun 4 23:13:42 1997 From: vblasch at gwdg.de (Volker Blaschke) Date: Mon Mar 7 07:30:01 2005 Subject: Quantification of T-cell infiltrate Message-ID: <33963CF6.25D9@gwdg.de> Dear fellow researchers, I need to quantify the T-cell infiltrate in biopsies and thought about CD3 as a marker. Now, since I am using quantitative RT-PCR, I am wondering which of the chains is best for this purpose? Are all equally useful? Thanks for the input! Volker -- ------------------------------------------------------------ Dr. Volker Blaschke Dept. of Dermatology vblasch@gwdg.de Georg-August-University Tel. xx49-551-396410 von-Siebold-Str. 3 (then have me paged) D-37075 Goettingen Germany From mdalton at worldnet.att.net Wed Jun 4 23:34:45 1997 From: mdalton at worldnet.att.net (Mark Dalton) Date: Mon Mar 7 07:30:01 2005 Subject: Homemade Chemiluminescent Protocols? References: <338EFCA7.41C6@risotto.mit.edu> Message-ID: <339641E5.819@worldnet.att.net> I tried this once it was much less sensitive than super signal from Pierce Chemicals. By the way Pierce is 1/2 the price of ECL from Amersham, and is more sensitive. I suggest you try the homemade stuff for yourself but make sure you do the controls the chemicals were very cheap around $20. I found protocol in the Red Book. It is listed in the other replies. Good Luck Mark Thomas Cameron wrote: > > Company kits end up costing $3-5 per western blot which is 90% of the > cost of the whole procedure. > > People must have some good homemade HRP (and AlkPhos) Chemiluminescent > Detection protocols or references? > > Could you send me one or point me to it? Thanks. > > -- > Thomas Cameron From frank.mullens at mcmail.vanderbilt.edu Mon Jun 2 16:57:35 1997 From: frank.mullens at mcmail.vanderbilt.edu (Frank Mullens) Date: Mon Mar 7 07:30:01 2005 Subject: plasmid DNA prep using CsCl-EtBr Message-ID: <339341CF.7F8D@mcmail.vanderbilt.edu> I have been troubled recently with my plasmid DNA prep using CsCl gradient method. I have noticed that there were significant amount of EtBr stuck to the DNA in some preparations (but not in all cases). EtBr seemed bound to DNA very tight and tolerated repeat extraction using 1-butanol, even when I titrated pH to 10.00 in an attempt to neutralize the positive group on EtBr. I wonder if these molecules somehow covelently bound to the plasmid DNA. I am not certain how these residual EtBr molecules may do to my experiment. Will they mess up my measurement of DNA concentration? cause toxicity once being introduced to cells? Can someone experienced offer me some hint on these questions and how to avoid this problem? Kink From flefever at ix.netcom.com Wed Jun 4 23:05:06 1997 From: flefever at ix.netcom.com (F. Frank LeFever) Date: Mon Mar 7 07:30:01 2005 Subject: Route of immunisation???? References: <338E908D.C9E@alpha2.curtin.edu.au> Message-ID: <5n5dti$bh5@dfw-ixnews5.ix.netcom.com> >>Hello everyone, >> I am pretty confuse here about the route of immunisation!!! I am >>currently working with a mouse model where I take my protein antigen in >>Freund's adjuvant.... and immunised the victim via intraperitoneal route >>.... in the hope of stimulating a good systemic response as well as good >>T-cells reponse.... >> However, I have been receiving advice from people (immunologists) >>and found that ip. immunisation is not a good way to go about if I want >>a good systemic/T-cells reponse..... They all recommended subcutaneous >>injection or intradermal injection.... >> Problem is I am so used to ip. route immunisation and my work have >>been sort of based on ip. immunisation.... and things have not been >>working very well for me... and on the other hand, if I change the route >>of immunisation.. then my work is sort of down the drain!!!! >> Does anyone have any insight to this!!!!????? Please!!!?? > >>Thank you!!!! > >>Sincerely, >>Grace Ho PhD student >>Curtin University of Technology >>Western Australia >>Email: EHO17@alpha1.curtin.edu.au > > >If the only reason you have to continue with i.p. immunization is that you >are used to it, maybe you should review your entire approach to research. > Omar O. Barriga Maybe Omar should review HIS approach to research. My inference is that she does not want to change the route IN THE MIDDLE OF A STUDY. Besides the merely "formal" consideration that one should not change details of procedure from subject to subject, there is the very real possibility that route may affect potency in ways you don't imagine. Don't have the reference right here, but I believe Bruce McEwen (current pres., Society for Neuroscience) was one author on recent paper (POSSIBLY in Proc. Nat. Acad. Sci) on impact of stress on immunization. One route may be more stressful than the other (and not just for the researcher!). Frank LeFever New York Neuropsychology Group From rjjensen at inav.net Wed Jun 4 23:14:27 1997 From: rjjensen at inav.net (Robert J Jensen) Date: Mon Mar 7 07:30:01 2005 Subject: Homemade Chemiluminescent Protocols? References: <338EFCA7.41C6@risotto.mit.edu> <339318A0.270E@bilbo.bio.purdue.edu> <5n12ht$prs@falcon.le.ac.uk> <5n2er6$n0m@dfw-ixnews8.ix.netcom.com> <5n47to$188@falcon.le.ac.uk> Message-ID: <33963D23.42B2@inav.net> Over the years I have had very good luck with chemicals from Sigma. In my book they are a top notch comapny. rjj From hk-miami at ix.netcom.com Wed Jun 4 19:01:15 1997 From: hk-miami at ix.netcom.com (HK) Date: Mon Mar 7 07:30:01 2005 Subject: Homemade Chemiluminescent Protocols? References: <338EFCA7.41C6@risotto.mit.edu> <339318A0.270E@bilbo.bio.purdue.edu> <5n12ht$prs@falcon.le.ac.uk> <5n2er6$n0m@dfw-ixnews8.ix.netcom.com> <5n47to$188@falcon.le.ac.uk> Message-ID: <5n4vkb$st0@sjx-ixn4.ix.netcom.com> x-no-archive: yes In <5n47to$188@falcon.le.ac.uk> "Dr E. Buxbaum" writes: > >hk-miami@ix.netcom.com(HK) wrote: > >>Maybe it wasn't the luminol...Maybe you mixed or measured something >>incorrectly, since you said that you only tried it once. >>I buy Luminol from Sigma...it works just fine. In a side-by-side >>test of my solutions and the ones in the Amersham ECL kit, my >>solutions work equally as well. > >I would not say things like this if I hadn't done the ovious tests. In >general I had quite a few problems with Sigma's chemicals over the years, >for example SDS which was insoluble in water, inactive enzyme... Looks >like a quality control problem to me. ----------- I'm not sure what a more oBvious test would be than testing it side by side with the reagents I was trying to duplicate and seeing that both worked equally well. What other test would you suggest? Helene > From swede at biodec.wustl.edu Thu Jun 5 15:42:19 1997 From: swede at biodec.wustl.edu (Marci Swede) Date: Mon Mar 7 07:30:01 2005 Subject: Homemade Chemiluminescent Protocols? References: <338EFCA7.41C6@risotto.mit.edu> <339318A0.270E@bilbo.bio.purdue.edu> <5n12ht$prs@falcon.le.ac.uk> <5n2er6$n0m@dfw-ixnews8.ix.netcom.com> <5n47to$188@falcon.le.ac.uk> <5n4vkb$st0@sjx-ixn Message-ID: <5n78bb$kdf$1@newsreader.wustl.edu> 4.ix.netcom.com> Some attributes got messed up here--x the complaint was about Sigma's luminol not working. The request was for testing if the failure was for Sigma luminol or some other reason that batch didn't work. HK (hk-miami@ix.netcom.com) wrote: : x-no-archive: yes : In <5n47to$188@falcon.le.ac.uk> "Dr E. Buxbaum" writes: : > : >hk-miami@ix.netcom.com(HK) wrote: : > : >>Maybe it wasn't the luminol...Maybe you mixed or measured something : >>incorrectly, since you said that you only tried it once. : >>I buy Luminol from Sigma...it works just fine. In a side-by-side : >>test of my solutions and the ones in the Amersham ECL kit, my : >>solutions work equally as well. : > : >I would not say things like this if I hadn't done the ovious tests. In : >general I had quite a few problems with Sigma's chemicals over the : years, : >for example SDS which was insoluble in water, inactive enzyme... Looks : >like a quality control problem to me. : ----------- : I'm not sure what a more oBvious test would be than testing it side by : side with the reagents I was trying to duplicate and seeing that both : worked equally well. What other test would you suggest? : Helene : > From u7x11ai at sunmail.lrz-muenchen.de Fri Jun 6 06:16:46 1997 From: u7x11ai at sunmail.lrz-muenchen.de (Dr. Sigrid Nikol) Date: Mon Mar 7 07:30:01 2005 Subject: Seek for an antibody Message-ID: <3397F19E.1387@sunmail.lrz-muenchen.de> Hi ! I am seeking an antibody against the bacterial neo-resistance gene product, usually used in eukaryotic expression vectors for selection in G418. Is there a way to get it ? Your suggestions and comments will be greatly appreciated. Alexander Maier From huckcho at pop.jaring.my Fri Jun 6 09:12:10 1997 From: huckcho at pop.jaring.my (Huckcho) Date: Mon Mar 7 07:30:01 2005 Subject: Website on suspected Coxsackie outbreak in Sarawak, Malaysia Message-ID: <5n95rq$25q@news2.jaring.my> Updates on suspected Coxsackie outbreak in Sarawak, Malaysia is available at http://www.jaring.my/jkns/outbreak/virus1.htm Huckcho From fof1 at chclu.chemie.uni-konstanz.de Fri Jun 6 10:14:53 1997 From: fof1 at chclu.chemie.uni-konstanz.de (Frank O. Fackelmayer) Date: Mon Mar 7 07:30:01 2005 Subject: Dissolve Zinc Chloride in Cell Culture Medium References: <01bc70f7$cfe11900$a5628bd0@default> Message-ID: In article <01bc70f7$cfe11900$a5628bd0@default>, "Suen Yick Keung" wrote: > I am going to test the possible inhibitory effect of zinc ions on apoptosis > of murine macrophage cell line. It is well known that zinc can inhibit > apoptosis in many cell types and I found there are many papers showing > reduction of apoptosis of culture cells in the presence of zinc chloride. > At the beginning of my experiment, I faced the most fundamental but > important problem: the solubility of zinc chloride in neutral pH culture > medium. Obviously, the zinc chloride cannot dissolve in the medium when > the pH is near neutral (around pH 7.2). No paper (as far as I have > obtained) mentioned this (may be it is too simple). And I was unable to > find the solution from any chemistry books. > > Do anyone know the answer ? Please Email to me. > > Thanks !! > > Y.K.Suen > > Email : yksuen@netvigator.com Try zinc sulfate, it dissolves up to 1M (at least). Hope this helps, Frank -- Dr. Frank O. Fackelmayer Division of Biology University of Konstanz D-78434 Konstanz Germany From yksuen at netvigator.com Fri Jun 6 10:47:04 1997 From: yksuen at netvigator.com (Suen Yick Keung) Date: Mon Mar 7 07:30:01 2005 Subject: Anti-DNase I and DNase II Message-ID: <01bc7291$0cc39560$c76b8bd0@default> Hi, Do anyone know any commercial supply of anti mouse DNase I and DNase II antibody ? Thank you for your attentions. Y.K.Suen. yksuen@netvigator.com From internetalias at mayo.edu Fri Jun 6 10:59:24 1997 From: internetalias at mayo.edu (Hongyu Yang) Date: Mon Mar 7 07:30:01 2005 Subject: human T cell lines without TCR Message-ID: <5n9c4s$d6k$1@tribune.mayo.edu> Hi, There. Does anyone know if there is a Human T cell line without the T cell receptor? I want use such a cell line to transfect my desired T cell receptor constructs. The only known Human T cell line without TCR alpha chain is a mutant Jurkat cells. but I don't know if there is a cell without both the Alpaha and beta chain. any comments and suggestions are highly appreciated. Hongyu From dmullins at vt.edu Fri Jun 6 09:30:38 1997 From: dmullins at vt.edu (David Warren Mullins) Date: Mon Mar 7 07:30:01 2005 Subject: interleukin designations Message-ID: What is the offical (Int. Union Imm. Soc.) interleukin designation upto? Is it IL-18? At the AAI meeting in San Fran, some people were referring to IL-18, and others were not stating that it's "not official." Also, can anyone tell me where to locate this information in the future? Many thanks, David ****************************************************************************** David W. Mullins Ph.D. Candidate Microbiology and Immunology Section, Department of Biology Virginia Polytechnic Institute and State University (Virginia Tech) 2119 Derring Hall Blacksburg, VA 24061-0406 voice: (540) 231-8933 fax: (540) 231-9307 http://www.vt.edu:10021/D/dmullins From devvlin at darwin.stanford.edu Sun Jun 8 19:23:59 1997 From: devvlin at darwin.stanford.edu (Brian Devlin) Date: Mon Mar 7 07:30:01 2005 Subject: IL-4 positive CD3-ve cells Help? References: <5n6mv9$221@info.abdn.ac.uk> Message-ID: <339B4D1C.5F00@darwin.stanford.edu> Good staining on CD3 positive and negative? Do you mean that it stains all cells? If thats the case, then something is thouroughly messed up. If you can't see a negative and a positive population for a stain, then its a worthless stain. From devvlin at darwin.stanford.edu Sun Jun 8 19:25:34 1997 From: devvlin at darwin.stanford.edu (Brian Devlin) Date: Mon Mar 7 07:30:02 2005 Subject: Is there a way to deminish IL-2 excess, without disrupting T-cell and NK cell activity? From a novice immunologist. References: <5n6v15$mkt@nccn3.nccn.net> Message-ID: <339B4D7B.3601@darwin.stanford.edu> anti IL-2 antibodies would be the common method (if they are available). From vim at usa.net Sat Jun 7 08:18:08 1997 From: vim at usa.net (Victoria) Date: Mon Mar 7 07:30:02 2005 Subject: Using a drop of blood to check CANCER!!! References: <01bc7293$7fcc84c0$LocalHost@jaring> Message-ID: <33995F90.1C17@usa.net> Chau Huang Kuan wrote: > > Dear everybody, > E. Excel international have successfully found a > method of using a drop of blood to check whether you got cancer or not. We > already apply the patent of this machine. Next year, 1998, every hospital > in the world will buy this machine from E. Excel. You will find a label on > that machine " made in E. Excel". Surprise!!! > This is really a amazing news.. Do you know that we can check out the > cancer > before it become serious? How many life we can save? snip... This is wonderful....however blood test for cancer is not new. CA125 is one. In my personal experience they is not always indicative. I had cancer and my blood screens (and pap smears) were all clear. According to the tests, I had no cancer. My reality was, I did have cancer. Victoria vim@usa.net From hkchua at pc.jaring.my Fri Jun 6 21:02:47 1997 From: hkchua at pc.jaring.my (Chau Huang Kuan) Date: Mon Mar 7 07:30:02 2005 Subject: Using a drop of blood to check CANCER!!! Message-ID: <01bc7293$7fcc84c0$LocalHost@jaring> Dear everybody, E. Excel international have successfully found a method of using a drop of blood to check whether you got cancer or not. We already apply the patent of this machine. Next year, 1998, every hospital in the world will buy this machine from E. Excel. You will find a label on that machine " made in E. Excel". Surprise!!! This is really a amazing news.. Do you know that we can check out the cancer before it become serious? How many life we can save? Another most important thing is all of our products have been proved that will be useful to prevent cancer. E. Excel has found the main reason of suffering from cancer. It's because our body cannot repair DNA which has been damaged by cancer cells. After the experiment, we found that all of our products have the ability to repair DNA, that means that we can prevent the cancer permanently. E. Excel International : http://www.geocities.com/hotsprings/8854/ Chau Huang Kuan Email: hkchua@pc.jaring.my URL: http://www.geocities.com/collegepark/union/1649/ From rom at hedda.uio.no Sun Jun 8 08:07:01 1997 From: rom at hedda.uio.no (Rolf Melheim) Date: Mon Mar 7 07:30:02 2005 Subject: Allergy Solution Message-ID: <1997060815070190219@xyplex05.uio.no> Allergy Solution Open letter to everyone interested: 15 years ago I became allergic, which for periods has been a hell. The doctor concluded after testing that I was over-sensible to birch, asp and alder - and I went every winter through a desensibilation program with injections of pollen extracts. During the worst allergy season in spring I also had to use antihistamines. But for a years I generally suspected other things to be the main reason for the increase of allergy problems - in many urban societies. Among things I suspected were substances in the food. This spring I have tested myself more thoroughly and has done the following, terrible conclusion: It is the drinking water! When I only drink water I buy on bottles (spring water), even for cooking coffee, and use no water from the tap, every trace of my allergy problem is gone! What's the main culprit I don't know, but one may suspect some chemicals used by the water treatment plants. If these chemicals may reach milk or other foodstufs I don't know. If also asthmatics may be helped by avoiding tap water I don't know, but it should be worth a try! Best regards - with more vitality Rolf Melheim Torvbakkgata 2 C 0550 Oslo Norway rolfme@forsok.vgs.no This far I have got 3 comments: 1. Rolf: That allergies to flouride and other chemicals used to treat public drinking water occure is well documented. Also, I'm not familar with how they process drinking water in Norway, so I don't know what they might be putting in it. Stagger Lee Stagger-lee@usa.net 2. Hi An even more common reason would surely be chlorine? Best wishes Alexa alexa@pcug.org.au 3. Subject: Reply to Allergy Solution Date: Thu, 05 Jun 1997 15:19:55 -0500 From: Jo Ann Faber To: "Rolf Melheim" Thank you for sharing your allergy solution with us. I have not come across any published information linking allergies and asthma with tap water. We have a member of the College in Oslo, Norway, (Kjell Aas, M.D., Voksentoppen Allergy & Asthma Institute) and I am forwarding your open e-mail letter to him. Sincerely, Jo Ann Faber ==================================== American College of Allergy Asthma & Immunology ACAAI Web Page: http://allergy.mcg.edu ==================================== From monk at postech.ac.kr Sun Jun 8 03:50:02 1997 From: monk at postech.ac.kr (Seung woo Lee) Date: Mon Mar 7 07:30:02 2005 Subject: Is there anyone who have an experience of CTL assay with rat lymphocyte ? Message-ID: <339b263a.8256999@news.postech.ac.kr> Hi guys! I had hard times to detect CTL response to specific antigen with rat (Buffalo rat; RT1b haplotype). I'm gonna use the hepatoma cell line, but I think it isn't good idea. So is there anyone who have an experience of CTL assay with rat lymphocyte ? I'll appreciate any idea to solve this problem. Thanks! From blethrow at cats.ucsc.edu Thu Jun 5 13:11:08 1997 From: blethrow at cats.ucsc.edu (Justin Blethrow) Date: Mon Mar 7 07:30:02 2005 Subject: Need diptheria toxin DNA for immunotoxin Message-ID: <3397013B.3FD2@cats.ucsc.edu> Hello all. An associate of mine is trying to make an immunotoxin project work on a shoestring budget. If anyone would be willing to send him DTA sequence, it would save a couple hundred dollars and would be enormously helpfull. Thaks in advance- Justin Blethrow From dennis_goos at mindlink.net Sat Jun 7 15:00:28 1997 From: dennis_goos at mindlink.net (dennis_goos@mindlink.net) Date: Mon Mar 7 07:30:02 2005 Subject: Using a drop of blood to check CANCER!!! References: <01bc7293$7fcc84c0$LocalHost@jaring> Message-ID: <3399badd.150314024@news.mindlink.net> "Chau Huang Kuan" wrote: >:|Dear everybody, >:| E. Excel international have successfully found a >:|method of using a drop of blood to check whether you got cancer or not. We >:|already apply the patent of this machine. Next year, 1998, every hospital >:|in the world will buy this machine from E. Excel. You will find a label on >:|that machine " made in E. Excel". Surprise!!! >:| This is really a amazing news.. Do you know that we can check out the >:|cancer >:|before it become serious? How many life we can save? >:| Another most important thing is all of our products have been proved that >:|will be useful to prevent cancer. E. Excel has found the main reason of >:|suffering from cancer. It's because our body cannot repair DNA which has >:|been >:|damaged by cancer cells. After the experiment, we found that all of our >:|products have the ability to repair DNA, that means that we can prevent the >:|cancer permanently. >:| E. Excel International : http://www.geocities.com/hotsprings/8854/ >:| >:|Chau Huang Kuan >:|Email: hkchua@pc.jaring.my >:|URL: http://www.geocities.com/collegepark/union/1649/ >:| >:| >:| And where do we read the peer reviews on this ? From paulroda at epix.net Sat Jun 7 11:55:39 1997 From: paulroda at epix.net (Paul I. Roda, M.D., F.A.C.P.) Date: Mon Mar 7 07:30:02 2005 Subject: Using a drop of blood to check CANCER!!! References: <01bc7293$7fcc84c0$LocalHost@jaring> Message-ID: <3399928B.50464246@epix.net> Chau Huang Kuan wrote: > Dear everybody, > E. Excel international have successfully found a > method of using a drop of blood to check whether you got cancer or > not. We > already apply the patent of this machine. Next year, 1998, every > hospital > in the world will buy this machine from E. Excel. You will find a > label on > that machine " made in E. Excel". Surprise!!! > This is really a amazing news.. Do you know that we can check > out the > cancer > before it become serious? How many life we can save? > Another most important thing is all of our products have been > proved that > will be useful to prevent cancer. E. Excel has found the main reason > of > suffering from cancer. It's because our body cannot repair DNA which > has > been > damaged by cancer cells. After the experiment, we found that all of > our > products have the ability to repair DNA, that means that we can > prevent the > cancer permanently. > E. Excel International : http://www.geocities.com/hotsprings/8854/ > > Chau Huang Kuan > Email: hkchua@pc.jaring.my > URL: http://www.geocities.com/collegepark/union/1649/ Wonderfull, another source of shoulder pork and ham on this net. First of all, DNA is not damaged by cancer cells, but damage to DNA is just one mechanism that leads to cancer. Other mechanisms include defects in inherited genes which modulate cell growth (oncogenes such as p53 and bcr -1), enviromental signals that cause metaplasia (tobacco, fat -- derived estrogens), and failure of senescent cells to undergoe apoptosis (programmed cell death). As far as a single test to detect cancer -- 30 years ago CEA was thought to be the test. It's helpful in known cancer cases, but isn't sensitive enough to be used as a screen. Ten years ago, a researcher published magnetic resonance data (never confirmed). Five years ago, everyone thought that CA-125 screening could detect early ovarian cancer. However, anything which irritated the pelvis could cause a mild elevation while a higher cutoff misses some early cases. In other words, I doubt you have the "gold standard" single test for cancer (and yes, I checked out your web page. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://iubio.bio.indiana.edu/bionet/mm/immuno/attachments/19970607/198b194a/attachment.html From devvlin at darwin.stanford.edu Sun Jun 8 19:38:46 1997 From: devvlin at darwin.stanford.edu (Brian Devlin) Date: Mon Mar 7 07:30:02 2005 Subject: Route of immunisation???? References: <338E908D.C9E@alpha2.curtin.edu.au> Message-ID: <339B5091.74E7@darwin.stanford.edu> "victim"? From paris at merck.com Mon Jun 9 12:18:52 1997 From: paris at merck.com (paris) Date: Mon Mar 7 07:30:02 2005 Subject: Need help! for parisitic illness in Thailand References: <5n01sf$4q0@dfw-ixnews6.ix.netcom.com> Message-ID: <339C3AFC.42DA@merck.com> > David J. Mike I would also consider animal parasites in this case since it does happen to be thailand. Have him also contact a veterinarian that may be able to look into these parasites. Most parasites can also cross over into humans and may have been entered through the GI tract (eaten) if he has GI problems. Have the doctors or vets consider nematodes such as Haemonchus, Trichostrongylus, or Ostertagi which are common to Cattle. Since it doesn't seem to be in his lungs I would rule out any dog or cat parasites. Anyway its a suggestion. Paris The contents of this message express only the sender's opinion. This message does not necessarily reflect the policy or views of my employer, Merck & Co., Inc. All responsibility for the statements made in this Usenet posting resides solely and completely with the sender. From paris at merck.com Mon Jun 9 12:11:20 1997 From: paris at merck.com (paris) Date: Mon Mar 7 07:30:02 2005 Subject: OUTBREAK OF ACUTE VIRUS INFECTION IN SARAWAK, MALAYSIA. References: <33934871.4A65@pc.jaring.my> Message-ID: <339C3938.493A@merck.com> > > Please contact : > > Sarawak Health Department > Email : JKNS2@po.jaring.my > Tel: 60-82-256566 Fax: 60-82-424959 Were the children located in any type of damp living conditions, that would preclude mold growth? There was an outbreak similar to what you describe reported here in the US in Ohio, it turned out not to be viral but rather a mold that was present in the homes of these children. Adults were not affected only growing children between the ages of 2 months and 5 years, and not all within the same radius. Could be a lead that you may not have thought about. Good luck, Paris The contents of this message express only the sender's opinion. This message does not necessarily reflect the policy or views of my employer, Merck & Co., Inc. All responsibility for the statements made in this Usenet posting resides solely and completely with the sender. From paris at merck.com Mon Jun 9 12:37:19 1997 From: paris at merck.com (paris) Date: Mon Mar 7 07:30:02 2005 Subject: Using a drop of blood to check CANCER!!! References: <01bc7293$7fcc84c0$LocalHost@jaring> Message-ID: <339C3F4F.1CF2@merck.com> After the experiment, we found that all of our > products have the ability to repair DNA, that means that we can prevent the > cancer permanently. > E. Excel International : http://www.geocities.com/hotsprings/8854/ > > Chau Huang Kuan > Email: hkchua@pc.jaring.my > URL: http://www.geocities.com/collegepark/union/1649/ Out for the Quik Buck, aren't we? Hmmmmmm........ The contents of this message express only the sender's opinion. This message does not necessarily reflect the policy or views of my employer, Merck & Co., Inc. All responsibility for the statements made in this Usenet posting resides solely and completely with the sender. From nobody at cc.unp.ac.za Mon Jun 9 09:01:08 1997 From: nobody at cc.unp.ac.za (nobody@cc.unp.ac.za) Date: Mon Mar 7 07:30:02 2005 Subject: Wanted: anti rat IgE / anti rabbit IgE Message-ID: Hi all, I would be most grateful if somebody would be willing to part with some of their anti-rat-IgE or anti-rabbit-IgE. Please let me know if you have some availible. Many thank's in advance, Rory Morty e-mail: MortyR@biochem.unp.ac.za From name at see.message.body Mon Jun 9 11:11:28 1997 From: name at see.message.body (Nigel C. Eastmond) Date: Mon Mar 7 07:30:02 2005 Subject: LPS -> IFN-g? Message-ID: <339C2B30.4EA@see.message.body> Hi, I have a problem with some of my expts, and am wondering if anyone can help. Unfortunately, it would be foolish of me to detail any protocols here, however some insight into one key question would be of enourmous help: Let's suppose you administered ip. LPS. Would you expect to get an rise in plasma/peritoneal IFN-g? If anyone wishes to answer this question in a more interactive way, then please email me directly as instructed below. Yours, Nige. -- Nigel C. Eastmond, Dept. Pharmacology, University of Liverpool. WWW: http://www.liv.ac.uk/~nce/> Mail: nce@liv.ac.uk If you took all the nephrons in your kidneys, and laid them out across the college quad', you'd be dead. From paris at merck.com Mon Jun 9 12:29:54 1997 From: paris at merck.com (paris) Date: Mon Mar 7 07:30:02 2005 Subject: goats and complement References: <970603232912_677702572@emout09.mail.aol.com> <5n3vlb$jbv$1@geraldo.cc.utexas.edu> Message-ID: <339C3D92.44A5@merck.com> WILLIAM J MANDY wrote: > > In article <970603232912_677702572@emout09.mail.aol.com>, CURRYBROWN@aol.com says: > > Rick: Apparently goats and sheep have problems with unusually high > levels of anti-complimentary activity. You may be better off with a > different assay if possible. What are you testing? > > Bill ( mandywj@mail.utexas.edu ) > > > > >Does anyone have experience with measuring complement in goat sera? Also are > >there companies and/or universities that have a working complement assay for > >goats? > > > >Rick I believe the University of Colorado is working with goat sera. The contents of this message express only the sender's opinion. This message does not necessarily reflect the policy or views of my employer, Merck & Co., Inc. All responsibility for the statements made in this Usenet posting resides solely and completely with the sender. From paris at merck.com Mon Jun 9 12:24:37 1997 From: paris at merck.com (paris) Date: Mon Mar 7 07:30:02 2005 Subject: Veterinary Immunology References: <0017000001005968000002L082*@MHS> Message-ID: <339C3C55.30F@merck.com> Cienkus,Keith wrote: > > Does anyone know of information relating to complement testing of goats > and/or know of any companies that perform this type of testing? My e-mail > address is cienkkr.add@notes.abbott.com Can I ask what type of complement testing you are inquiring about? I do know of one company that will be working with goats but the testing is done outside at a university. Please respond via newsgroup, Paris and no it isn't merck. The contents of this message express only the sender's opinion. This message does not necessarily reflect the policy or views of my employer, Merck & Co., Inc. All responsibility for the statements made in this Usenet posting resides solely and completely with the sender. From hkchua at pc.jaring.my Sun Jun 8 20:04:09 1997 From: hkchua at pc.jaring.my (Chau Huang Kuan) Date: Mon Mar 7 07:30:02 2005 Subject: Founder of Using a drop of blood to check CANCER!!! Message-ID: <01bc7466$ee2c5420$LocalHost@jaring> Dr. Jau-Fei Chen's profile Obtained Bachelor Degree in Microbiology and Chemistry at the age of 19. Obtained Master Degree in Microbiology, with emphasis in Immunology and Biochemistry at the age of 21. Obtained Ph. D. Degree in Microbiology at the age of 26, taught upper immunology 512 for both Master and PhD program in Brigham Young University. Founded E. Excel International at Utah, U.S.A. in 1987. Invited to present research paper at the conference of International Federation of Immunologist in Washington D. C. in 1987 and 1988. Selected to receive the Global Overseas Chinese Best Young Person Award in 1992. Selected to receive Martin De La Cruz Award. The Highest Honor in the sixth International Congress on Traditional and Folk Medicine held in 1992. Selected to receive the Second Annual Model of Overseas Chinese Youth Entrepreneur in 1993. Selected as one of the 100 most influential Chinese in U.S.A. in 1994. Invited to attend "The Asia Outstanding Chinese New Year Eve Dinner" hosted by U.S.A. President Bill Clinton in 1996. Received "outstanding woman of the year" award in 1996. The Senate of California state designated March 8, 1996 as "Jau-Fei Chen's Day". Received first place award in thesis categories in the Third Annual Conference of World Traditional Medicine in 1996. Named as one of Ten Outstanding Young Americans for 1997 by the United States Junior Chamber of Commerce at their annual congress on January 9-11, 1997 in Washington DC. Further Information, please visit Nutritional Immunology Home Page. URL : http://www.geocities.com/hotsprings/8854/ Regards, -- Chau Huang Kuan e-mail: hkchua@pc.jaring.my From monk at postech.ac.kr Sun Jun 8 21:50:47 1997 From: monk at postech.ac.kr (Seung woo Lee) Date: Mon Mar 7 07:30:02 2005 Subject: Is there anyone who have an experience of CTL assay with rat? Message-ID: <339c205e.4790154@news.postech.ac.kr> Hi guys! I had hard times to detect CTL response to specific viral antigen in rat (Buffalo rat; RT1b). The major problem is the target cell. I used the hepatoma cell line of BUF rat, but this cell has high percentage of spontaneous Cr release and I wonder whether this cell has right component such as MHC, accessory molecules etc. Is there anyone who have an experience of CTL assay with rat or give me any idea to solving this problem? Seung woo Lee monk@postech.ac.kr From ghermans at luc.ac.be Tue Jun 10 03:06:00 1997 From: ghermans at luc.ac.be (Guy Hermans) Date: Mon Mar 7 07:30:03 2005 Subject: T cell epitope prediction References: Message-ID: In article , bunce@lincoln.ac.nz (Michael Bunce) wrote: > Does anyone know where I can find some online algorithms to map out possible T > cell epitopes from a primary sequence? I have found Epiplot, but would like > to get hold of TSites and some other programs that are mentioned in the > literature. I sent an e-mail to a few groups concerning Tsites a few weeks ago. I'll mail you a copy on private e-mail - I don't want to overload usenet servers all over the planet with big attachments. By the way, there is a WWW site (www.dejanews.com) that will locate 'old' e-mail concerning virtually any topic. You can even check the author's e-mail profile! A little bit too big brother to my taste, but helpfull if you want to know who you're dealing with. And no, I'm not connected to these people. But this site would have helped you - that's all I wanted to say. The T-sites program is on it's way to you right now. Only Mac format available, no source code available at the moment to cross-compile, unfortunately. See you, Guy -- Guy Hermans, PhD student Ms research Unit Immunology research group Dr. L. Willems-Institute Dept. of Physiology, LUC University Campus University Campus B-3590 Diepenbeek B-3590 Diepenbeek Belgium Belgium Voice ++32(0)11/26.92.07 Fax ++32(0)11/26.92.09 From jalcorn427 at aol.com Tue Jun 10 11:22:53 1997 From: jalcorn427 at aol.com (JAlcorn427) Date: Mon Mar 7 07:30:03 2005 Subject: Need anti-protein kinase G Message-ID: <19970610162200.MAA12647@ladder02.news.aol.com> I am currently conducting follow up experimentation on the effects of testosterone on corpus cavenosal SMC. I would like to do western blot analysis if I can obtain Ab. Please e-mail me with any information. Thank you, John From fclement at allserv.rug.ac.be Tue Jun 10 06:19:28 1997 From: fclement at allserv.rug.ac.be (frederic clement) Date: Mon Mar 7 07:30:03 2005 Subject: Wanted:KCG Message-ID: <339D383E.6481@allserv.rug.ac.be> Can anyone tell me where to buy Kathon CG (a bacteriocide) ? Please, mail me on this address: evkersch@allserv.rug.ac.be From bunce at lincoln.ac.nz Tue Jun 10 14:20:12 1997 From: bunce at lincoln.ac.nz (Michael Bunce) Date: Mon Mar 7 07:30:03 2005 Subject: T cell epitope prediction Message-ID: Does anyone know where I can find some online algorithms to map out possible T cell epitopes from a primary sequence? I have found Epiplot, but would like to get hold of TSites and some other programs that are mentioned in the literature. Thanks in advance, Mike Bunce AVSG Lincoln University New Zealand Email: Bunce@lincoln.ac.nz From kshreder at znet.com Tue Jun 10 17:01:26 1997 From: kshreder at znet.com (Kevin Shreder) Date: Mon Mar 7 07:30:03 2005 Subject: The Antibody Resource Page Message-ID: <339DCEB6.4303@znet.com> The Antibody Resource Page (ARP) has recently moved to a new URL. The ARP is divided up into 7 sections: 1. Educational Resources - links to pages on antibodies that will interest the novice and expert alike 2. Online Databanks and Databases - links to scientific databases in the area of sequence analysis and hybridoma work 3. Online Journals 4. How to Find an Antibody - a section for ways to find commercial sources (online or otherwise) of antibodies. If you are a researcher who works with antibodies, you cannot afford to miss this section. 5. Online Companies - a large list of online companies (over 90) that sell antibodies or antibody related products. There is also a section for companies that are not online. 6. Miscellaneous - links to various immunological and biotechnology webpages 7. Antibody Gallery - a new addition to the ARP. This is a section where researchers can donate pictures of antibodies for educational purposes. If you have something to donate, please contact me. The ARP is designed for both beginners and experts who are looking for information about antibodies. I am always looking for new links, so if you know of something, please contact me. Or just contact me to let me know what you think of the page! The URL for the Antibody Resource Page is: http://www.antibodyresource.com/ Kevin Shreder, Ph.D. kshreder@znet.com From mkoziel at WEST.BIDMC.HARVARD.EDU Wed Jun 11 00:02:50 1997 From: mkoziel at WEST.BIDMC.HARVARD.EDU (margaret koziel MD) Date: Mon Mar 7 07:30:03 2005 Subject: Euthanasia effects on CTL? Message-ID: <339D6E4E.8E@west.bidmc.harvard.edu> Hello all. I am currently starting some murine experiments after several years of doing only human studies. Specifically, I am interested in immune responses in mice that express a viral protein in the liver. Most of the labs I know use cervical dislocation alone (seems like that's because they have done it for 15 years). Our animal care committee strongly disapproves of cervical dislocation as a method of euthanasia. However, in the course of writing protocols for our animal care committee, I reviewed some of the literature on methods of euthanasia and the effect of different methods on cellular proliferation and CTL assays. I couldn't find much, but a couple of studies showed alterations of both Th and CTL responses when other anesthestics were combined with cervical dislocation (eg methoxyflurane, pentobarb, or CO2). Does anyone have any experience with different methods of murine euthanasia? If there were any differences, were they significant or trivial? Also, since one of the readouts of the experiments is hepatitis, has anyone using methoxyflurane encountered this as a significant problem? Thanks, Margaret Koziel Beth Israel Deaconess Med. Ctr. Boston MA From shelly1811 at aol.com Wed Jun 11 17:37:49 1997 From: shelly1811 at aol.com (Shelly1811) Date: Mon Mar 7 07:30:03 2005 Subject: Urea Breath Test Message-ID: <19970611223701.SAA02512@ladder02.news.aol.com> Hi, I'm a student writting a paper on the UBT (C13). I have found information all over the place as to the sensitivity/specificity. I would like to hear from anyone using the test to let me know of and false positives or the reliability of the test in the low range...2.4 - 4.0. Thanks for your time. shelly1811@aol.com From betts at BOISDARC.TAMU-COMMERCE.EDU Wed Jun 11 13:33:38 1997 From: betts at BOISDARC.TAMU-COMMERCE.EDU (Gordon Betts) Date: Mon Mar 7 07:30:03 2005 Subject: Need a progestin inhibitor Message-ID: <3.0.1.32.19970611133601.006e5e40@boisdarc.tamu-commerce.edu> Does anyone know where I can find RU486 or another progestin inhibitor? thanx gordon ====================================================== J. Gordon Betts, Ph.D. Department of Biological Sciences Texas A&M University - Commerce Commerce, TX 75429-3011 formerly East Texas State University Voice 903/886-5369 FAX 903/886-5991 E-Mail: betts@boisdarc.tamu-commerce.edu ====================================================== From dhavilan at IMM2.IMM.UTH.TMC.EDU Wed Jun 11 12:17:28 1997 From: dhavilan at IMM2.IMM.UTH.TMC.EDU (David L. Haviland, Ph.D.) Date: Mon Mar 7 07:30:03 2005 Subject: Euthanasia Message-ID: <3.0.32.19970611121853.006ecce4@imm2.imm.uth.tmc.edu> At 09:26 6/11/97 -0400, Jeff Frelinger wrote: >Several years ago we did a couple of side by side expts (of course using >different mice) and the metaphane responses seemed to be lower. It was >enough that we were convinced. It is my personal opinion that the ACUC >are usually more concerned with esthetics for the experimenter than >anything else. Somehow sticking a mouse in a jar is better than >breaking their necks. Jeff: I agree with your conclusion as far as results. From jfrelinger at atlas.niaid.nih.gov Wed Jun 11 08:26:12 1997 From: jfrelinger at atlas.niaid.nih.gov (Jeff Frelinger) Date: Mon Mar 7 07:30:03 2005 Subject: Euthanasia Message-ID: <339EA774.7B07@atlas.niaid.nih.gov> Several years ago we did a couple of side by side expts (of course using different mice) and the metaphane responses seemed to be lower. It was enough that we were convinced. It is my personal opinion that the ACUC are usually more concerned with esthetics for the experimenter than anything else. Somehow sticking a mouse in a jar is better than breaking their necks. Jeff Frelinger From brett at BORCIM.WUSTL.EDU Wed Jun 11 01:52:23 1997 From: brett at BORCIM.WUSTL.EDU (brett) Date: Mon Mar 7 07:30:03 2005 Subject: Hot Young *** Sucking Teens * Message-ID: <199706101524.KAA23291@borcim.wustl.edu> >Check out this site, it has tons of Nude Teenagers ****ing >and Sucking ****. > >Are you ready to *** all over a teenagers face? Then Check out: > > > http://www.nasty-schoolgirls.com > > http://www.nasty-schoolgirls.com > > http://www.nasty-schoolgirls.com ALRIGHT, I CALL AGAIN FOR MODERATION. OTHERWISE, I'LL STOP SUBSCRIBING. WHO WANTS THIS GARBAGE DELIVERED *DAILY* TO THEIR MAILBOX?!? Brett Lindenbach Program in Immunology Washington University - St Louis brett@borcim.wustl.edu From janhan at cbs.dtu.dk Thu Jun 12 06:44:55 1997 From: janhan at cbs.dtu.dk (Jan Hansen) Date: Mon Mar 7 07:30:03 2005 Subject: T cell epitope prediction References: Message-ID: <339FE137.6201@cbs.dtu.dk> Michael Bunce wrote: > > Does anyone know where I can find some online algorithms to map out possible T > cell epitopes from a primary sequence? I have found Epiplot, but would like > to get hold of TSites and some other programs that are mentioned in the > literature. > > Thanks in advance, > > Mike Bunce > AVSG > Lincoln University > New Zealand > Email: Bunce@lincoln.ac.nz Please see: Parker tool http://www-bimas.dcrt.nih.gov/molbio/hla_bind/index.html epimatrix tool http://www.epimatrix.com/hiv -- Jan Hansen Center for Biological Sequence Analysis Department of Physical Chemistry The Technical University of Denmark Building 206 DK-2800 Lyngby Denmark Phone: +45 4525 2485 Fax: +45 4593 4808 E-mail: janhan@cbs.dtu.dk WWW: http://www.cbs.dtu.dk/janhan/homepage.html From Oviedo-Orta at cardiff.ac.uk Thu Jun 12 15:37:28 1997 From: Oviedo-Orta at cardiff.ac.uk (Ernesto Oviedo Orta) Date: Mon Mar 7 07:30:03 2005 Subject: Post Message-ID: I am a young MD, with 5 years of experience in immunology research. I looking for a PhD studenship or an immunology related post in UK. I have also experience in Molecular and Cell Biology techniques. CV on resquest. Please if you are interest write me to the following address. Ernesto Oviedo Orta, M.D Medical Research Council Intercellular Signaling Team, Department of Medical Biochemistry, University of Wales Coll. of Medicine, Heath Park, Cardiff CF4 4XN, U.K e-mail: oviedo-orta@cardiff.ac.uk Tel: 01222-747747 Exts: 2284, 2802 Direct Line: 01222-742284, 01222-742802 Fax: 01222-766276 From levy at uab.edu Fri Jun 13 18:02:30 1997 From: levy at uab.edu (David N. Levy) Date: Mon Mar 7 07:30:03 2005 Subject: What HLA type is Jurkat cell line? Message-ID: <5nsji6$7ej@maze.dpo.uab.edu> Anyone know what the HLA type of the T lymphocytic cell line Jurkat is? Thanks. David N. Levy University of Alabama at Birmingham Birmingham, AL 35294-0007 levy@uab.edu From hkchua at pc.jaring.my Fri Jun 13 12:45:19 1997 From: hkchua at pc.jaring.my (Chau Huang Kuan) Date: Mon Mar 7 07:30:03 2005 Subject: Company of using BLOOD to check CANCER!!! Message-ID: <01bc781d$c41dc000$LocalHost@jaring> Professional Achievements of E. Excel International In 1987, Dr. Jau-Fei Chen took her science of Nutritional Immunology one step further by founding E. Excel International, Inc. -- a company specializing in the formulation and manufacture of nutritionally superior herbal food products -- as a way to provide people with the nutrition that they need to maintain a properly functioning immune system. Dr. Chen currently serves as President of E. Excel International, Inc. E. Excel International is a worldwide research and manufacturing company, with facilities and offices around the world. E. Excel's products are the finest in the world, and every formulation has been created not from ancient manuscripts, but from the latest scientific research and technology combined with traditional herbal knowledge. By retesting, rethinking, researching, and then rejecting any shortcomings until every formulation is perfect, E. Excel has developed the most nutritionally excellent products available. The mission of E. Excel is to give the gift of health through its line of products, and the gift of knowledge through continued research in Nutritional Immunology. Each member of the E. Excel research team and staff is dedicated to a high level of excellence, exemplified by none other than Dr. Chen, and to living up to what the company name stands for -- double the excellence! It is not mere happenstance that E. Excel headquarters is located in Springville, Utah. On the contrary, Dr. Chen carefully selected the 15 acre lot on which she built her offices and manufacturing facility because of its pristine environment. Nestled at the base of the breathtaking Wasatch mountains, among the clearest water and freshest air, E. Excel headquarters is located on beautiful wetlands where the land, water, and wildlife are preserved an protected from the pollution of modern day life -- making it one of the choicest locations available. Meticulous care is taken at E. Excel headquarters to manufacture the highest quality products in the industry. The facility conforms to strict governmental regulations, including wall and floor sanitation. The manufacturing facility is separate from all other facilities to ensure purity, and each product is packaged and handled with the utmost care. E. Excel has designed its own, highly technical machinery to produce these products without compromising the essential nutrients inside plant foods. This equipment is also used to ensure the lowest possible microbial exposure levels keeping E. Excel products free from contamination. Daily research is performed in E. Excel's own in-house laboratory. The products are tested at each stage of manufacture to guaranty the highest quality available. Each member of the E. Excel personnel is well-trained and conscientious about their responsibilities. It is by the combined efforts of scientists, engineers, computer specialists, and many others that E. Excel products are produced and distributed. In addition to its extensive manufacturing plant, E. Excel maintains a spacious warehouse and shipping facility to ensure its products will be readily available to meet the global demand. The entire E. Excel product line is the result of years of research performed by Dr. Chen to ensure enhanced immune system function through maximum nutritional benefits. With each passing year, E. Excel continues to expand. It has truly been the vehicle by which Dr. Chen is accomplishing her goal of providing better health to all people. Further Information, please visit Nutritional Immunology Home Page. URL : http://www.geocities.com/HotSprings/8854/ Regards, -- Chau Huang Kuan e-mail: hkchua@pc.jaring.my From derek.gray at surgery.oxford.ac.uk Fri Jun 13 18:16:07 1997 From: derek.gray at surgery.oxford.ac.uk (Derek Gray) Date: Mon Mar 7 07:30:03 2005 Subject: Euthanasia effects on CTL? References: <339D6E4E.8E@west.bidmc.harvard.edu> Message-ID: <33A1D4B7.2BD4@surgery.oxford.ac.uk> margaret koziel MD wrote: > > Hello all. I am currently starting some murine experiments after several > years of doing only human studies. Specifically, I am interested in > immune responses in mice that express a viral protein in the liver. > Most of the labs I know use cervical dislocation alone (seems like > that's because they have done it for 15 years). Our animal care > committee strongly disapproves of cervical dislocation as a method of > euthanasia. However, in the course of writing protocols for our animal > care committee, I reviewed some of the literature on methods of > euthanasia and the effect of different methods on cellular proliferation > and CTL assays. I couldn't find much, but a couple of studies showed > alterations of both Th and CTL responses when other anesthestics were > combined with cervical dislocation (eg methoxyflurane, pentobarb, or > CO2). Does anyone have any experience with different methods of murine > euthanasia? If there were any differences, were they significant or > trivial? Also, since one of the readouts of the experiments is > hepatitis, has anyone using methoxyflurane encountered this as a > significant problem? > > Thanks, > > Margaret Koziel > Beth Israel Deaconess Med. Ctr. > Boston MA Margaret, Will your animal care committee accept CO2 narcosis as an acceptable euthanasia method for mice? This is rapid, (1-2 minutes) appears to cause no suffering and involves no use of synthetic anaesthetic agent. It is accepted by the UK Home Office as a schedule 1 killing method, and I would suspect we have the strictest licencing procedures in the World. Derek Gray Nuffield Department of Surgery Oxford From ptbmb at liverpool.ac.uk Fri Jun 13 05:56:49 1997 From: ptbmb at liverpool.ac.uk (Bernadette Brooks) Date: Mon Mar 7 07:30:04 2005 Subject: CLIP ab Message-ID: <33A12771.5B09@liverpool.ac.uk> Can anyone tell me if there is an antibody available to CLIP ? Thanks From r1623 at erols.com Thu Jun 12 19:47:56 1997 From: r1623 at erols.com (Rick and Marilyn Schuman) Date: Mon Mar 7 07:30:04 2005 Subject: goat complement Message-ID: <33A098BC.4E73@erols.com> Does anyone know of a source of newborn goat complement? Any suggestions appreciated. Thanks, Rick Schuman From plextech at ix.netcom.com Fri Jun 13 22:38:03 1997 From: plextech at ix.netcom.com (Victor Holland) Date: Mon Mar 7 07:30:04 2005 Subject: Beckman Biomek Interface Message-ID: <01bc7873$db553d60$bce1d3c6@dell-laptop> Has anyone had any experience interfacing RS232 serial communication instruments to Beckman Biomek 2000s? I would like to put a ICN Titertek Multidrop on mine. It has a fairly good set of RS232 commands. I just need to figure out how to get the Biomek to talk to it. Any help would be appreciated. Thank you. - Victor plextech@ix.netcom.com From friedr at med.unc.edu Fri Jun 13 22:30:34 1997 From: friedr at med.unc.edu (Randall H. Friedline) Date: Mon Mar 7 07:30:04 2005 Subject: T cell epitope prediction References: Message-ID: <33A2105A.C48F9DDA@med.unc.edu> Michael Bunce wrote: > Does anyone know where I can find some online algorithms to map out > possible T > cell epitopes from a primary sequence? I have found Epiplot, but > would like > to get hold of TSites and some other programs that are mentioned in > the > literature. > > Thanks in advance, > You can try Ken Parker's HLA site: http://bimas.dcrt.nih.gov/molbio/hla_bind/ -- Cheers, Randy friedr@med.unc.edu " I am Homer of Borg. (.sig stolen from someone) Resistance is..........Mmmmmm..donuts!!" From rjjensen at inav.net Sat Jun 14 09:59:51 1997 From: rjjensen at inav.net (Robert J Jensen) Date: Mon Mar 7 07:30:04 2005 Subject: goat complement References: <33A098BC.4E73@erols.com> Message-ID: <33A2B1E7.7BFA@inav.net> Have you tried Pierce Chemical, or Sigma Chemical? From paimm at netgate.net Sun Jun 15 12:13:47 1997 From: paimm at netgate.net (PAIMM) Date: Mon Mar 7 07:30:04 2005 Subject: Symposium: Translation & Stability of mRNA Message-ID: <33A422CB.419B@netgate.net> San Francisco Symposium '97 Translation & Stability of mRNA October 12-14, 1997 Airport Hilton San Francisco, CA USA ORGANIZED BY: Joe Harford PhD National Cancer Institute Michael Katze PhD University of Washington James W. Larrick MD PhD Palo Alto Institute of Molecular Medicine Confirmed Speakers Joel Belasco, New York University Thomas Dever, NICHD Tom Donahue, Indiana University Gideon Dreyfuss, Univ. Pennsylvania Ellie Ehrenfeld, NIH Stan Fields, Univ. of Washington Elizabeth Goodwin, Northwestern Univ. Joe Harford, National Cancer Institute Matthias Hentze, EMBL,Heidelberg Allan Jacobson, Univ. of Massachusetts Michael Katze, Univ. of Washington Jack Keene, Duke University Ruth Lehmann, New York University Lynne Maquat, Roswell Park Cancer Inst. William Merrick, Case Western Reserve Joel D. Richter, Worcester Foundation Jeff Ross, McArdle Laboratory Alan Sachs, UC Berkeley Paul Schimmel, MIT Daniel R. Schoenberg, Ohio State Univ. Robert Singer, A. Einstein College of Med. Nahum Sonenberg, McGill University Palo Alto Institute of Molecular Medicine 2462 Wyandotte Street Mountain View, CA 94043, USA TEL: 415--694-1420; FAX: 415--694-7717 E-mail: paimm@netgate.net www.pano.com/paimm From uncleal at uvic.ca Sun Jun 15 10:18:16 1997 From: uncleal at uvic.ca (Uncle Al Schwartz) Date: Mon Mar 7 07:30:04 2005 Subject: Laboratory Robotics Show References: <01bc7944$c2e5ac20$5c775ecf@dell-laptop> Message-ID: <33A407B8.138@uvic.ca> Andy Zaayenga wrote: > > DON'T MISS IT! Thirty-one vendors will present their latest technologies > and services to The Laboratory Robotics Interest Group on Vendors Night > this Thursday, June 19 from 5:00 to 8:30 pm! Admission is free - and there > will be free hors d'oeuvre courtesy of the vendors. A cash bar will be > available. This is a great opportunity for you to meet with your peers in > the laboratory automation community. Come on out and enjoy an evening with > the LRIG! [massive mercantile snip] What happens to your products' software, firmware, and embedded microprocessesors when the calendar flips to January 2000? Those elegant automated doodads might be a very short-term investment. They tend to track validation and calibration dates, you know. ISO 9002! Not just a screwup, but a perfectly documented SOP FUBAR screwup. -- Alan "Uncle Al" Schwartz UncleAl0@ix.netcom.com ("zero" before @) uncleal@uvic.ca (summer only, cAsE-sensitive!) http://www.ultra.net.au/~wisby/uncleal.htm (Toxic URL! Unsafe for children, Democrats, and most mammals) "Quis custodiet ipsos custodes?" The Net! From tcckd at pc.jaring.my Sun Jun 15 12:44:14 1997 From: tcckd at pc.jaring.my (Tay Chon Chong) Date: Mon Mar 7 07:30:04 2005 Subject: Company of using BLOOD to check CANCER!!! References: <01bc781d$c41dc000$LocalHost@jaring> Message-ID: <01bc79b3$b380c980$e1d58ea1@jaring> Chau Huang Kuan wrote in article <01bc781d$c41dc000$LocalHost@jaring>... > Professional Achievements of > E. Excel International > It is mainly commercialized rather than Nutritional! I known some of the E. Excel International 'representatives' who claim their products can activates human immunity system. Scientific medicines are harmful their are save and can cure all types of illness..... I saw patient's DM deteriorated, hypertension worsening, pocket more empty because money is sucked by them. From tedl at top.spamblock.net Sun Jun 15 18:22:37 1997 From: tedl at top.spamblock.net (Ted Leonard) Date: Mon Mar 7 07:30:04 2005 Subject: Company of using BLOOD to check CANCER!!! References: <01bc781d$c41dc000$LocalHost@jaring> <01bc79b3$b380c980$e1d58ea1@jaring> Message-ID: In article <01bc79b3$b380c980$e1d58ea1@jaring>, "Tay Chon Chong" wrote: > Chau Huang Kuan wrote in article > <01bc781d$c41dc000$LocalHost@jaring>... > > Professional Achievements of > > E. Excel International > > > > It is mainly commercialized rather than Nutritional! > > I known some of the E. Excel International 'representatives' who claim > their products can activates human immunity system. Scientific medicines > are harmful their are save and can cure all types of illness..... > > I saw patient's DM deteriorated, hypertension worsening, pocket more empty > because money is sucked by them. Who gives a damn what your spammed crap is or ain't! Take it out of sci.bio.herp. -- Ted Leonard tedl@top.net http://www.top.net/tedl/standingbear Signature space for rent. Inquire within. From Oz at upthorpe.demon.co.uk Sun Jun 15 15:38:23 1997 From: Oz at upthorpe.demon.co.uk (Oz) Date: Mon Mar 7 07:30:04 2005 Subject: Company of using BLOOD to check CANCER!!! References: <01bc781d$c41dc000$LocalHost@jaring> <01bc79b3$b380c980$e1d58ea1@jaring> Message-ID: In article <01bc79b3$b380c980$e1d58ea1@jaring>, Tay Chon Chong writes > > >Chau Huang Kuan wrote in article ><01bc781d$c41dc000$LocalHost@jaring>... >> Professional Achievements of >> E. Excel International >> > >It is mainly commercialized rather than Nutritional! > >I known some of the E. Excel International 'representatives' who claim >their products can activates human immunity system. Scientific medicines >are harmful their are save and can cure all types of illness..... > >I saw patient's DM deteriorated, hypertension worsening, pocket more empty >because money is sucked by them. > For some strange reason I am not surprised by this post. Whoever would have guessed that Excel Int might not be completely bona-fide? Could it be that real companies with good products don't seem to need to promote their products with self-laudatory posts to newsgroups? No, surely not. -- 'Oz "Is it better to seem ignorant and learn, - or seem wise and stay ignorant?" From zaayenga at lab-robotics.org Sat Jun 14 23:33:11 1997 From: zaayenga at lab-robotics.org (Andy Zaayenga) Date: Mon Mar 7 07:30:04 2005 Subject: Laboratory Robotics Show Message-ID: <01bc7944$c2e5ac20$5c775ecf@dell-laptop> DON'T MISS IT! Thirty-one vendors will present their latest technologies and services to The Laboratory Robotics Interest Group on Vendors Night this Thursday, June 19 from 5:00 to 8:30 pm! Admission is free - and there will be free hors d'oeuvre courtesy of the vendors. A cash bar will be available. This is a great opportunity for you to meet with your peers in the laboratory automation community. Come on out and enjoy an evening with the LRIG! Participating Vendors ---------------------------------------- Ace Glass- Custom Labware Argonaut Technologies, Inc. - Organic Compound Synthesis The Automation Partnership Beckman / Sagian BioDot Inc. (possible) Bohdan Automation Brandel Incorporated Corning / Costar CRS Robotics CyberLab Incorporated Denville Scientific Dynex (formerly Dynatech) - MLX-1000 luminometer EG&G Wallac Incorporated Hewlett Packard Hudson Control Group Inc. InnovaSystems Labman Automation Ltd Matrix Technologies Corporation Millipore Corporation New England Nuclear Packard Instrument Company Perceptive Biosystems Qiagen Robbins Corporation Source For Automation Tecan TomTec TiterTek / Labrepco Incorporated Waters Corporation Whatman Zymark Corporation ------------------------------------------------------- The Laboratory Robotics Interest Group Topical Group of the North Jersey American Chemical Society June 1997 Meeting The officers of the Laboratory Robotics Interest Group are pleased to announce: The Third Annual Vendor's Night Date: Thursday, June 19, 1997 Place: The Morris Room The Embassy Suites Hotel Route 202 Parsippany, New Jersey Time: 5:00 to 8:30 PM Pre-Registration: not required Extensive hors d'oeuvre, courtesy of the vendors, will be available as well as a cash bar. The proceeds from this vendor funded exhibition are also used to finance mailings and pay for various costs of running the group. In this way the LRIG can operate without collecting dues. Please support the group by attending this informative and entertaining meeting. Thirty-one vendors of laboratory automation software and hardware will be present, demonstrating their latest products and services. Last year's Vendor's Night was extremely successful and we hope to surpass that turnout. For vendor registration and more information contact Ed Kanczewski, Vice Chairman, or any of the LRIG officers listed below. There will be rooms available for attendees who wish to stay overnight. Note: If you are receiving this mailer in paper form, please get your email address to us if possible! The Officers: Chairman: Dennis France dennis.france@pharma.novartis.com Novartis (201)503-6030 Vice Chairman: Ed Kanczewski kanczee@aa.wl.com Warner-Lambert (201)540-6479 Secretary: Andy Zaayenga zaayenga@ix.netcom.com Zymark Corporation (908)302-1038 Treasurer: William Haller bhaller@ompus.jnj.com Ortho-McNeil Pharmaceutical (908)218-6341 The LRIG web site is still evolving! Check us out at http://www.lab-robotics.org We offer meeting announcements, a message board, and career opportunities. There are also many links to industry related meetings and conferences, automation web sites, newsgroups, manufacturers, consultants, and our members' companies. Email is becoming very important to us as we try to keep mailing costs down. If you have an email address, please either log on to the web site and leave us a message or send email to zaayenga@ix.netcom.com. DIRECTIONS: Embassy Suites Hotel 909 Parsippany Blvd. Parsippany, NJ 07054 Tel: (201)334-1440 Fax:(201)402-1188 From anastas at MAIL.CYBERLINK.BG Sun Jun 15 15:39:53 1997 From: anastas at MAIL.CYBERLINK.BG (Anastas Pashov) Date: Mon Mar 7 07:30:04 2005 Subject: Route of immunisation???? Message-ID: <33A4514B.7096@mail.cyberlink.bg> Dear Grace, Consider the following "rules": - i.d. -> Th1 - i.p. -> Th2 - CFA -> Th1 - IFA -> Th2 I hope this is of any help. Best regards! Anastas Pashov From Slides at webtv.net Sat Jun 14 20:04:37 1997 From: Slides at webtv.net (Rose Kingsley) Date: Mon Mar 7 07:30:05 2005 Subject: Help! I Got Pinworms From My Kids! Message-ID: <5nvf35$4le$1@newsd-106.bryant.webtv.net> Hello Everyone, I got pinworms while I was teaching a special education class 2 years ago. I have taken Mebendazole, garlic (synthetic and whole cloves) and I still have them. I started my treatments late in my infestation. I did not know what I had for about a year. I just experienced anal itching intermittently for the first year, then it intensified. I started the mebendazole at that point. How can I get rid of these parasites? Has anybody out there tried the product called Clear? Does it really work? Any recommendations on treatments or medications would be appreciated. Please reply by E-Mail, if possible. Address E-Mail to: Slides@WebTV.Net Thank you in advance for any help you can give me. Sincerely, Rose Kingsley From teitelba at aecom.yu.edu Mon Jun 16 08:37:33 1997 From: teitelba at aecom.yu.edu (Rachel Teitelbaum) Date: Mon Mar 7 07:30:05 2005 Subject: Route of immunisation???? References: <33A4514B.7096@mail.cyberlink.bg> Message-ID: More rules: -mucosally administered, Th1 -other routes, Th2 On 15 Jun 1997, Anastas Pashov wrote: > Dear Grace, > > Consider the following "rules": > - i.d. -> Th1 > - i.p. -> Th2 > - CFA -> Th1 > - IFA -> Th2 > I hope this is of any help. > > Best regards! > > Anastas Pashov > > From R.Batrla at DKFZ-Heidelberg.de Mon Jun 16 14:38:35 1997 From: R.Batrla at DKFZ-Heidelberg.de (Richard Batrla) Date: Mon Mar 7 07:30:05 2005 Subject: dendritic cells: MANUAL Message-ID: <33A596A6.7BD7@DKFZ-Heidelberg.de> Hi, does anyone know of a manual for working with dendritic cells? I would be grateful to get these information. Thanx From nightfires at geocities.com Mon Jun 16 10:13:52 1997 From: nightfires at geocities.com (Sigel Bolverk VinDuran) Date: Mon Mar 7 07:30:05 2005 Subject: The Realm Message-ID: <5o3lc5$igg$4@node2.frontiernet.net> Come and visit this great new web site http://www.geocities.com/Athens/Acropoplis/5113/ From jyoung at camelot.bradley.edu Mon Jun 16 15:18:42 1997 From: jyoung at camelot.bradley.edu (James Young) Date: Mon Mar 7 07:30:05 2005 Subject: Acetylcholine antibodies Message-ID: <5o4732$jop@camelot.bradley.edu> Does anyone know where an enterprising research student could acquire some monoclonal antibodies against acetylcholine? Is there even such thing out there? Thanks in advance Jim Young jyoung@camelot.bradley.edu From ttera at libra.bekkoame.or.jp Mon Jun 16 03:51:17 1997 From: ttera at libra.bekkoame.or.jp (Tetsuo Teranishi) Date: Mon Mar 7 07:30:05 2005 Subject: How to obtain the p53 ? Message-ID: <33A4FE82.46FE@libra.bekkoame.or.jp> Please teach me how to obtain the p53 proteins (wild and mutant). 1, Factory or laboratory name 2, Adress, Phone and Fax number From frauwirt at mendel.Berkeley.EDU Mon Jun 16 15:18:49 1997 From: frauwirt at mendel.Berkeley.EDU (Ken Frauwirth BioKen) Date: Mon Mar 7 07:30:05 2005 Subject: CLIP ab References: <33A12771.5B09@liverpool.ac.uk> Message-ID: <5o4739$62k@agate.berkeley.edu> In article <33A12771.5B09@liverpool.ac.uk>, Bernadette Brooks wrote: >Can anyone tell me if there is an antibody available to CLIP ? >Thanks There are two antibodies that I know of which recognize human CLIP: CerCLIP (Peter Cresswell, Yale) detects CLIP free or bound to MHC Class II, but not whole Ii. 30-2 (Alexander Rudensky, UWash - Seattle) detects CLIP only in the context of the murine A(b) molecule. There is some cross-reactivity with murine CLIP, and it can also detect some of the larger Ii fragments (SLIP, LIP) bound to A(b). As far as I know, there is no monoclonal specifically against mouse CLIP, although the epitope for P4H5 overlaps with CLIP (P4H5 can detect whole Ii, but not CLIP-deleted fragments). I believe P4H5 is available from ATCC. Hope that helps, Ken Frauwirth -- Ken Frauwirth (MiSTie #33025) _ _ frauwirt@mendel.berkeley.edu |_) * |/ (_ |\ | http://www.ocf.berkeley.edu/~frauwirt/ |_) | () |\ (_ | \| DNRC Title: Chairman of Joint Commission on In-duh-vidual Affairs From michael at demon.demon.co.uk Tue Jun 17 04:40:05 1997 From: michael at demon.demon.co.uk (michael dalton) Date: Mon Mar 7 07:30:05 2005 Subject: Acetylcholine antibodies References: <5o4732$jop@camelot.bradley.edu> Message-ID: In article <5o4732$jop@camelot.bradley.edu>, James Young writes >Does anyone know where an enterprising research student could acquire >some monoclonal antibodies against acetylcholine? Is there even such >thing out there? > >Thanks in advance >Jim Young >jyoung@camelot.bradley.edu > I would have thought the acetyl choline molecule was too small, and too essential for there to be a possibility to make antibodies against them -- michael dalton From fclement at allserv.rug.ac.be Tue Jun 17 06:32:23 1997 From: fclement at allserv.rug.ac.be (frederic clement) Date: Mon Mar 7 07:30:05 2005 Subject: IgM-Elisa Message-ID: <33A675C5.6C02@allserv.rug.ac.be> Hello, Has anyone a good Elisa-protocol for the detection of antigen specific IgM's in human serum ? Is a blocking like BSA needed ? Has anyone experience with very good blockers ? Please, mail me on this address: evkersch@allserv.rug.ac.be From aronsev at post.tau.ac.il Tue Jun 17 08:45:14 1997 From: aronsev at post.tau.ac.il (Evgeny Arons) Date: Mon Mar 7 07:30:05 2005 Subject: tk luc plasmid Message-ID: <5o64da$e4o@mserv1.dl.ac.uk> Hi! Does anybody knows where I can find a map of "tk luc" plasmid? Please e-mail me with any information. Thanks, Evgeny Arons Tel Aviv University From eped at sn.no Tue Jun 17 18:48:19 1997 From: eped at sn.no (Bjoern K. Pedersen) Date: Mon Mar 7 07:30:05 2005 Subject: Antibodies on the NET Message-ID: <33A72243.F40@sn.no> There is a Norwegian company that has recently appeared on the Web: http://www.diatec.com They offer high quality Mabs for research use, and seems to be the first company to base the sales of such products entirely on the Internet. They accept order on the Web, they communicate with you directly through e-mail, they ship by DHL and they supply you Mabs at a reasonable price. So far they have only launched 2 products, but the list of forthcoming products seems promising. I recommend you visit their site! -- - Bj?rn Pedersen From stalib at ubmedg.buffalo.edu Tue Jun 17 11:24:14 1997 From: stalib at ubmedg.buffalo.edu (stalib@ubmedg.buffalo.edu) Date: Mon Mar 7 07:30:05 2005 Subject: Culturing Dendritic cells from CD34+ cells Message-ID: <866560631.10027@dejanews.com> I am a doctoral student at Roswell Park in Buffalo and am currently trying to culture Dendritic cells from CD34+ cells. I am using Iscove's DMEM media supplemented with 10% FBS and the following cytokines, 100ng/ml GM-CSF and stem cell factor and 50ng/ml of TNFalpha. I would like to know what conditions are best for culturing dendritic cells. For eg. media, serum type (human vs bovine), concentration of cytokines used, days of culturing, etc. Also how many cells should I start out iwht and how many cells will I get in the end. Any help is really appreciated. Thanks shaema -------------------==== Posted via Deja News ====----------------------- http://www.dejanews.com/ Search, Read, Post to Usenet From serge at influenza.spb.su Tue Jun 17 05:40:20 1997 From: serge at influenza.spb.su (Sergey Shevtsov) Date: Mon Mar 7 07:30:05 2005 Subject: immunology Message-ID: subscribe bionet.immunology From cwebster at broombio.demon.co.uk Tue Jun 17 10:06:19 1997 From: cwebster at broombio.demon.co.uk (Craig Webster) Date: Mon Mar 7 07:30:05 2005 Subject: CRP Measurements Message-ID: Is there any utility in measuring CRP levels below 10 mg/L. I believe there are some sensitive assays on the market that make this possible. Are there other comments about CRP measurements in general ? -- Craig Webster |Tel: 01245 514013 Senior Clinical Biochemist |Fax: 01245 514077 Broomfield Hospital |email: cwebster@broombio.demon.co.uk Chelmsford, Essex, UK |http://www.broombio.demon.co.uk/ From g.briars at mailbox.uq.edu.au Tue Jun 17 00:50:57 1997 From: g.briars at mailbox.uq.edu.au (Graham Briars) Date: Mon Mar 7 07:30:05 2005 Subject: Help! I Got Pinworms From My Kids! References: <5nvf35$4le$1@newsd-106.bryant.webtv.net> Message-ID: <5o58k1$dk2$1@nargun.cc.uq.edu.au> In article <5nvf35$4le$1@newsd-106.bryant.webtv.net>, Slides@webtv.net (Rose Kingsley) says: > > > >Hello Everyone, > >I got pinworms while I was teaching a special education class 2 years ago. I have taken Mebendazole, garlic (synthetic and whole cloves) and I still have > >Has anybody out there tried the product called Clear? Does it really work? Any recommendations on treatments or medications would be appreciated. Please r > >Thank you in advance for any help you can give me. > > Sincerely, > > Rose Kingsley > You could try piperazine. It sounds like reinfection. Did you treat your whole household in paralell From martha59 at TERRACOM.NET Mon Jun 16 19:11:19 1997 From: martha59 at TERRACOM.NET (Martha Reilly) Date: Mon Mar 7 07:30:05 2005 Subject: Message-ID: Subscribe immuno From sni1 at le.ac.uk Wed Jun 18 05:25:45 1997 From: sni1 at le.ac.uk (S.N. Imlach) Date: Mon Mar 7 07:30:05 2005 Subject: Why more HLA-DR ? Message-ID: <5o8d39$ka7@hawk.le.ac.uk> Why is more HLA-DR, in general, expressed on the surface of APC. According to my reading so far the same mechanisms for regulating the expression of HLA-DR used for DP and DQ. Does this mean that the promptor activity in DR is increased over the other Class II gene ? Does this overrepresentation of HLA-DR confer any immunological advantage ? Thanks in advance Stuart From sni1 at le.ac.uk Wed Jun 18 05:19:48 1997 From: sni1 at le.ac.uk (S.N. Imlach) Date: Mon Mar 7 07:30:05 2005 Subject: Why more HLA-DR ? Message-ID: <5o8co4$i9o@hawk.le.ac.uk> Why is it that in general more HLA-DR molecules are found on the surface of APC when according to my reading the mechanism for the expression of HLA Class II genes are the same. Does the overrepresentation of HLA-DR confer some advantage to the immune system? Thanks in advance From sni1 at le.ac.uk Wed Jun 18 05:13:26 1997 From: sni1 at le.ac.uk (S.N. Imlach) Date: Mon Mar 7 07:30:05 2005 Subject: Why more HLA-DR Message-ID: <5o8cc6