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[Zbrafish] Re: In Situ Hybridization in zebra fish embryo. Yolk sac turns black after fixation.

laraberas from gmail.com via zbrafish%40net.bio.net (by laraberas from gmail.com)
Mon Sep 26 17:38:23 EST 2016


El miércoles, 22 de junio de 2016, 12:20:54 (UTC-3), elh... from gmail.com escribió:
> I am performing In Situ Hybridization in zebra fish embryos and wonder why the yolk sac turns black after fixing them with 4% paraformaldehyde in PBS overnight at 4°C. I thought you could help me with this problem. I removed the chorions before fixation.

Hi, I am having the same problem. Did you find a solution? Thanks.



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