Hi everyone,
I tried recently to collect blood from adult zebrafish to measure some
coagulation parameters, but I failed to obtain more than 6 µl from a big,
old female. This was either with MS-222 or ice anesthesia. I collected the
blood with a 10µl tip, in the main dorsal blood vessel, after cutting the
tail of the anesthetized fish.
Could anyone advice me on how to obtain a larger blood volume (10 to 15 µL
would be fine)?
Thank you
Marie Winandy, PhD
Universite de Liege
Giga zebrafish platform
http://www.giga.ulg.ac.be/jcms/c_5418/en/giga-zebrafish-facilities
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