I have a question regarding the primer design for amplifying probe
templates containing an RNA polymerase promoter to produce RNA probe.
While designing the primers attached with T7 promoter sequence, just adding
the promoter sequence at 5' extremity of the primer is enough or a short
sequence of GAAAT should also be added before the promoter sequence? Is it
either 5'-TAATACGACTCACTATAGGGnnnnnnn-3' or
5'-gaatTAATACGACTCACTATAGGGnnnnnnn-3'? The template to be used for the PCR
Eagerly waiting for a reply
Thanks and Regards
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