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[Zbrafish] Re: Announcement of FOURTH Solicitation for NIH-funded Resource to Target Zebrafish Genes with Engineered Nucleases

Keith Joung via zbrafish%40net.bio.net (by keith.joung from zincfingers.org)
Sun Jan 6 18:50:56 EST 2013


Dear all,

Listed below are the 96 zebrafish genes and target sites for which we
will make FLASH-assembled TALENs for the community as part of our NIH-
sponsored initiative.  We hope to deposit the plasmids encoding these
TALENs with Addgene by February 2013.  We will write with updates as
we make those deposits.

Best,

Keith Joung
Randall Peterson
Joanna Yeh
Jeffry Sander
Deepak Reyon
Jon Foley
Massachusetts General Hospital

TALEN #	Targetsitename	TALEN (5' to 3')
1	ZebrafishCommunity-aak1-left	TCTTTGACTCCCGGAGAG
1	ZebrafishCommunity-aak1-right	TCCCCCTCCAGAACCTGG
2	ZebrafishCommunity-amh-left	TCCAGGCAAGATTTGGGC
2	ZebrafishCommunity-amh-right	TACGACCCAATCGCCACG
3	ZebrafishCommunity-arf6-left	TGGGGAAGATGCTTTCCA
3	ZebrafishCommunity-arf6-right	TCAATATTCTCATCTCC
4	ZebrafishCommunity-arnt1-left	TCCCGTCTCTGGCCATGA
4	ZebrafishCommunity-arnt1-right	TGCACACCGTTGGAATGA
5	ZebrafishCommunity-atg12-left	TTAATCAGTCGTTTGCGC
5	ZebrafishCommunity-atg12-right	TCAAAAAGCACACCAACT
6	ZebrafishCommunity-atg5-left	TAAAGTCAAGAAACATTT
6	ZebrafishCommunity-atg5-right	TTCCTCCACATCCTCTGC
7	ZebrafishCommunity-avpl-left	TGCTGTGTGTGCTCGCGC
7	ZebrafishCommunity-avpl-right	TTCTGGATGTAGCAGGCA
8	ZebrafishCommunity-Ccl2-left	TTTTCGTTTTCCACTATA
8	ZebrafishCommunity-Ccl2-right	TATGATAACTCTGAACCT
9	ZebrafishCommunity-cebpb-left	TGGAAGTGGCCGGTTTTT
9	ZebrafishCommunity-cebpb-right	TTAGTGCTGTGGAAAGCG
10	ZebrafishCommunity-CIT1-left	TGAAGTTTAAATATGGAG
10	ZebrafishCommunity-CIT1-right	TCGACTGCGGACAGATCT
11	ZebrafishCommunity-cited2-left	TGGTAGACCGCATGATGG
11	ZebrafishCommunity-cited2-right	TTCACAGCATCTGGGAAA
12	ZebrafishCommunity-ComplementFactorH-left	TGATTACATGTGAACCTC
12	ZebrafishCommunity-ComplementFactorH-right	TCCAAAAGGAGGTGGACA
13	ZebrafishCommunity-crispld2-left	TATCAGGAGGAGCTAGAA
13	ZebrafishCommunity-crispld2-right	TTCGGACAGGAGCGTCGG
14	ZebrafishCommunity-dachc-left	TCCAGACCTGGCAGACCT
14	ZebrafishCommunity-dachc-right	TGTCGGGTGATGTCACAT
15	ZebrafishCommunity-dbx1b-left	TCCCGTGCAAGGAATGAA
15	ZebrafishCommunity-dbx1b-right	TCCGTCAAAACAAGGGAA
16	ZebrafishCommunity-DGKE-left	TATGGTGTCAAACCACAG
16	ZebrafishCommunity-DGKE-right	TCATCGGTCAGACTGGAA
17	ZebrafishCommunity-Dlk1-left	TGGTGGCGCAGCAGAGGG
17	ZebrafishCommunity-Dlk1-right	TGATGTTCACGGTGGCC
18	ZebrafishCommunity-Dlk2-left	TTGGCCACGGAAAATGTG
18	ZebrafishCommunity-Dlk2-right	TCCCGGATCACACCTG
19	ZebrafishCommunity-drd4b-left	TTCACTTACAATGTTCCC
19	ZebrafishCommunity-drd4b-right	TAACAATGATAAGCAATA
20	ZebrafishCommunity-EMP2-left	TCAGGCAGTGCAGGCCAC
20	ZebrafishCommunity-EMP2-right	TCCCACACAGCACAAGAT
21	ZebrafishCommunity-eps15-left	TTGCCCATTGCCTATCGC
21	ZebrafishCommunity-eps15-right	TAGTGTTTCTGTTCCCAC
22	ZebrafishCommunity-esr1-left	TCAGTGGGATACTACCCG
22	ZebrafishCommunity-esr1-right	TCTGTAGATGTTCCTCGT
23	ZebrafishCommunity-fam123b-left	TGGAGATCGCCACAAGAT
23	ZebrafishCommunity-fam123b-right	TCTGACGATGGACCCCTC
24	ZebrafishCommunity-fgfbp1a-left	TGTCTCTCCGCGGAATTT
24	ZebrafishCommunity-fgfbp1a-right	TGTGAGACGCACGCGAGA
25	ZebrafishCommunity-foxl2-left	TAACGGCATGATACTCAT
25	ZebrafishCommunity-foxl2-right	TCGGTCTTTCTCCGCTGA
26	ZebrafishCommunity-FoxP1b-left	TCGGGGACAGAGGCGGCA
26	ZebrafishCommunity-FoxP1b-right	TGGGCGGAGCGCCGTTCT
27	ZebrafishCommunity-foxp3a-left	TGCTTCTTAATGCTACTG
27	ZebrafishCommunity-foxp3a-right	TGACTACTCCTGTTATCA
28	ZebrafishCommunity-Frizzled5-left	TCGGATTTTGGCTGCATG
28	ZebrafishCommunity-Frizzled5-right	TCTCCGAGCCCAGACAGT
29	ZebrafishCommunity-Fryl-left	TTCCAGGCGGTGCGTAAG
29	ZebrafishCommunity-Fryl-right	TCAGCCTCAGTTCCTTCA
30	ZebrafishCommunity-fscn2a-left	TAAATCACGAAAATCGAT
30	ZebrafishCommunity-fscn2a-right	TTGATCTTGAAACCAAAT
31	ZebrafishCommunity-GnIH-left	TGTAAGATGTCCTACTTC
31	ZebrafishCommunity-GnIH-right	TGCTCAGGATGCCGAGGG
32	ZebrafishCommunity-GnRH3-left	TTGGAGGTCAGTCTTTGC
32	ZebrafishCommunity-GnRH3-right	TTCCACCGGGAAGCCAAC
33	ZebrafishCommunity-gper-left	TGGAGGAGCAGACTACCA
33	ZebrafishCommunity-gper-right	TGCTCGGTGCCATTCACA
34	ZebrafishCommunity-hemogen-site1-left	TGGAAGACCCGTTGGAGA
34	ZebrafishCommunity-hemogen-site1-right	TCTGAATCTTTTATTTCA
35	ZebrafishCommunity-hemogen-site2-left	TGATTTGTTTGTTTGCT
35	ZebrafishCommunity-hemogen-site2-right	TCTCTGTCTCTGAGTCG
36	ZebrafishCommunity-hif1al-site1-left	TGTTTCAGCAGAGCCCCG
36	ZebrafishCommunity-hif1al-site1-right	TCCACTCCTTCCATCTCC
37	ZebrafishCommunity-hif1al-site2-left	TGCCCTCAGGACTTCTGC
37	ZebrafishCommunity-hif1al-site2-right	TATTGGAGACAGAAGCT
38	ZebrafishCommunity-hirip3-left	TGGCCAAAGAAAAGGACG
38	ZebrafishCommunity-hirip3-right	TTCTGCAGTTGAGCCACA
39	ZebrafishCommunity-hist2h3ca1-left	TCTGTTTGAGGACACCAA
39	ZebrafishCommunity-hist2h3ca1-right	TGATGGTGACCCTCTTGG
40	ZebrafishCommunity-hnrnpr-left	TGGATGCGGGTCTGCCCC
40	ZebrafishCommunity-hnrnpr-right	TGGAAGATGTTATCCAGG
41	ZebrafishCommunity-hnrnpu-left	TTAAGGTGAACGAGCTGA
41	ZebrafishCommunity-hnrnpu-right	TTGTCAGAGAGCTGCCGC
42	ZebrafishCommunity-hs3st1l1-left	TCTTCTGAGTTATGTGTT
42	ZebrafishCommunity-hs3st1l1-right	TGGAGGAGCGGCGTATGC
43	ZebrafishCommunity-hs3st1-left	TCAATCCTCTGTCCTGGT
43	ZebrafishCommunity-hs3st1-right	TAGCGCAGCATCAGGCGG
44	ZebrafishCommunity-Id4-left	TTTATTACAATGAAGGCC
44	ZebrafishCommunity-Id4-right	TAGAAGGAAGCTTATGAG
45	ZebrafishCommunity-IPIP27A-left	TGAACGAGAGGAGTGTGG
45	ZebrafishCommunity-IPIP27A-right	TTGTCTGGAGGAGAGTTG
46	ZebrafishCommunity-jarid2a-left	TGGGATCCCGTGGTCAGA
46	ZebrafishCommunity-jarid2a-right	TGAGAGGTAGAGCACCCG
47	ZebrafishCommunity-lhx1a-left	TTTATCGGACAAAGAAAC
47	ZebrafishCommunity-lhx1a-right	TCCAAGGTTCTGGTCATC
48	ZebrafishCommunity-lrrtm1-left	TCTAATTGGTCTGTACCT
48	ZebrafishCommunity-lrrtm1-right	TATCAGCCCAGGGGGCTT
49	ZebrafishCommunity-mak-left	TCAAGCAGCTGGGTGATG
49	ZebrafishCommunity-mak-right	TTGCTCTTCCCCATTAGA
50	ZebrafishCommunity-mapk14a-left	TCTTCTTGTGCTCTTATT
50	ZebrafishCommunity-mapk14a-right	TCAGCGCCCATAAGGTGA
51	ZebrafishCommunity-max-left	TGAGCGACAACGATGA
51	ZebrafishCommunity-max-right	TAACTATACTCACATC
52	ZebrafishCommunity-mchr1a-left	TGGCCATCTGTTTGGCCT
52	ZebrafishCommunity-mchr1a-right	TGAAGGATGTAATATGGA
53	ZebrafishCommunity-mef2aa-left	TGGGACGGAAGAAGATAC
53	ZebrafishCommunity-mef2aa-right	TGCCGGTTCCTCTCATCC
54	ZebrafishCommunity-meis2a-left	TGAGCTGGCTCACTACGG
54	ZebrafishCommunity-meis2a-right	TGGATCCCCGTACATCCC
55	ZebrafishCommunity-meis4.1a-left	TACGAGGATCTGGTCCAC
55	ZebrafishCommunity-meis4.1a-right	TCGGAGACGGGATCCCGA
56	ZebrafishCommunity-mrc1a-left	TGTTGTACAAGGGATCAG
56	ZebrafishCommunity-mrc1a-right	TTGGTCCCATACACCTGC
57	ZebrafishCommunity-NPVF-left	TTAGCCCTCGGCATCCTG
57	ZebrafishCommunity-NPVF-right	TGAGAGCCGTAACTTCAC
58	ZebrafishCommunity-nr5a2-left	TGGACTACTCCTACGACG
58	ZebrafishCommunity-nr5a2-right	TCTCCGCACACTGGACAC
59	ZebrafishCommunity-nup155-left	TCGCTGTCTGGCGTGTCC
59	ZebrafishCommunity-nup155-right	TCAGCGCGGGACCCTGCA
60	ZebrafishCommunity-NUPL2-left	TGGATAAACCCATCTCAG
60	ZebrafishCommunity-NUPL2-right	TGCCCCCTGCTGACGAGG
61	ZebrafishCommunity-ovol1-left	TGTTCCAGTTCCAGCGCA
61	ZebrafishCommunity-ovol1-right	TGCTCGCTGTGACATTTC
62	ZebrafishCommunity-oxtr-left	TTCAAGGATCAAGACTTT
62	ZebrafishCommunity-oxtrl-left	TGATCCTCTGGCCCATTA
63	ZebrafishCommunity-oxtrl-right	TCGTTTCCCCTACTTGAG
63	ZebrafishCommunity-oxtr-right	TGGTGGCGTTTCTGGATG
64	ZebrafishCommunity-park2-left	TGATCGTTTTCGTGCGGT
64	ZebrafishCommunity-park2-right	TCCAACTCCACCGGGAAC
65	ZebrafishCommunity-pcolce2b-left	TCCTCACATTTCGTTTCC
65	ZebrafishCommunity-pcolce2b-right	TCGTACCGACACAGGTTG
66	ZebrafishCommunity-pink1-left	TTTCCTCTCATAGTCAAT
66	ZebrafishCommunity-pink1-right	TTTACTGACATTTTGAGC
67	ZebrafishCommunity-Popdc2-left	TGAATTGGAGACAGCAGA
67	ZebrafishCommunity-Popdc2-right	TCAATGAAGAGTCGCCGC
68	ZebrafishCommunity-Popdc3-left	TCTCACTGTTAAGATTAT
68	ZebrafishCommunity-Popdc3-right	TATTCTCAGGTAAAAGAT
69	ZebrafishCommunity-prothymosinalphaa-left	TTATCATTCGCATCTCGT
69	ZebrafishCommunity-prothymosinalphaa-right	TGGGGTCTCGGAATAAAA
70	ZebrafishCommunity-prrx1b-left	TAATACACTTGCCAATTT
70	ZebrafishCommunity-prrx1b-right	TGCATAGCTAGAAGTCAT
71	ZebrafishCommunity-PurinergicreceptorY2-left	TTCAACGCATCAGTGAAT
71	ZebrafishCommunity-PurinergicreceptorY2-right	TGCTGCAGCTGTGTGCAG
72	ZebrafishCommunity-rassf7b-left	TGAAGGTGTGGGTGGATG
72	ZebrafishCommunity-rassf7b-right	TCTTCTGAGAGGCCACAG
73	ZebrafishCommunity-scrt2-left	TTTTCTGGTGCGTCTGTG
73	ZebrafishCommunity-scrt2-right	TCACCAGAAACGAGCGAG
74	ZebrafishCommunity-sema6dl-left	TGCTGATACTCCTGCTGG
74	ZebrafishCommunity-sema6dl-right	TCAGGAAAGCTCACAGCA
75	ZebrafishCommunity-Semaphorin6bb-left	TTGAAAAGTGCTGAGAAA
75	ZebrafishCommunity-Semaphorin6bb-right	TTTGTCTCCTAAATCAAA
76	ZebrafishCommunity-si:ch211-120j21.1-left	TGAACTGTCAGTCGGGAC
76	ZebrafishCommunity-si:ch211-120j21.1-right	TGAAAGAAAGCGCATAAA
77	ZebrafishCommunity-slc50a1-left	TTTCTGCAGCTGCTATCA
77	ZebrafishCommunity-slc50a1-right	TGAACATCCCGACGGTGA
78	ZebrafishCommunity-snrnp35-left	TCCGGTGGCGAAGGTGTA
78	ZebrafishCommunity-snrnp35-right	TGTGCCGTCAATGCTGCC
79	ZebrafishCommunity-snrnp48-left	TGTGTGAAACATCGTTTG
79	ZebrafishCommunity-snrnp48-right	TGAAGACACTGCATGCGC
80	ZebrafishCommunity-sox1a-left	TATAGCATGATGATGGAA
80	ZebrafishCommunity-sox1a-right	TGTTGGTTTGGGGTCCCG
81	ZebrafishCommunity-Sox2-left	TGGTGGGGTAGACTTTCG
81	ZebrafishCommunity-Sox2-right	TTTCCATCATGTTATACA
82	ZebrafishCommunity-Tac3b-left	TCCACGTGCTGCTGCTGC
82	ZebrafishCommunity-Tac3b-right	TCGAGGGCGCTCCGCGAG
83	ZebrafishCommunity-taok2b-left	TGCGGGCTGGGAGTCTCA
83	ZebrafishCommunity-taok2b-right	TCCTTGTAAAAAAGTTCC
84	ZebrafishCommunity-tcof-left	TAAAGACTCCTCCACCAG
84	ZebrafishCommunity-tcof-right	TCACTACTGGACACTGCA
85	ZebrafishCommunity-tet2(ENSDART00000113020)-left	TCAAGACCAGATACTATC
85	ZebrafishCommunity-tet2(ENSDART00000113020)-right
TTCAGTAGGTGGTAGCTG
86	ZebrafishCommunity-tet3(ENSDART00000137355)-left	TTGCTCCTTGGTTGCATC
86	ZebrafishCommunity-tet3(ENSDART00000137355)-right
TAATGAAGAAAATGTTTG
87	ZebrafishCommunity-tfe3a-left	TAAAATAGCCAGTCAGA
87	ZebrafishCommunity-tfe3a-right	TGAGGGCTGTGGTGGG
88	ZebrafishCommunity-tfe3b-left	TCGCTCAGCTCCGCCCT
88	ZebrafishCommunity-tfe3b-right	TGGCAGCGAGACGGAGAT
89	ZebrafishCommunity-tnfb-left	TGTCGGGGGAGTTTATCA
89	ZebrafishCommunity-tnfb-right	TGAAGAATTAACCGGTAC
90	ZebrafishCommunity-TRABD2A-left	TCAGCTGGAGCGCTTTTC
90	ZebrafishCommunity-TRABD2A-right	TTTGCTCTCACCATGAGT
91	ZebrafishCommunity-TTC19-left	TGCCAGGAGGAGCAGGGA
91	ZebrafishCommunity-TTC19-right	TCACTCATCAGGACCAGC
92	ZebrafishCommunity-Vangl1-left	TTCTCTGCCCAGGATAG
92	ZebrafishCommunity-Vangl1-right	TGTCAGTAAACCCAGC
93	ZebrafishCommunity-vasa-left	TGACTGGGAGGAAGATCA
93	ZebrafishCommunity-vasa-right	TCCAAATCCTGAGCTGCA
94	ZebrafishCommunity-Vrk2-left	TGTACTTGGCTGACTATG
94	ZebrafishCommunity-Vrk2-right	TGCTCGCCATTAGGACAG
95	ZebrafishCommunity-ywhai-left	TACAAGAATGTGGTGGGA
95	ZebrafishCommunity-ywhai-right	TGCTGGACACTACCCTCC
96	ZebrafishCommunity-zmynd11-left	TATGTTTGGTCGGCCATC
96	ZebrafishCommunity-zmynd11-right	TGTTGGCGATTTGTTTCT



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