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[Zbrafish] how to stop zebrafish heart beat

Castranova, Daniel (NIH/NICHD) [C] via zbrafish%40net.bio.net (by castrand from mail.nih.gov)
Wed Aug 14 15:27:35 EST 2013


Hi Weibin,

Have you considered ice?  By gradual chilling you may be able to slow down the heartbeat enough to get your images.  Some researchers have played around with the idea of using ice as a method for euthanasia, but in early life stages fish seem to recover.  Here is a nice paper that talks about it:

Anesthesia and Euthanasia in Zebrafish. Matthews and Varga, 2012. ILAR Journal. V53 Issue 2. Pp. 192-204.

Good luck,

Dan

Daniel Castranova
Aquatic Research Specialist
Charles River Laboratories - Contractor
Unit on Vertebrate Organogenesis
Program in Genomics of Differentiation, NICHD, NIH
Building 6B, Room 322, 6 Center Drive
Bethesda, MD 20892
(301)594-0904
castrand from mail.nih.gov

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From: <Zhou>, Weibin <weibinz from med.umich.edu<mailto:weibinz from med.umich.edu>>
Date: Tuesday, August 13, 2013 8:27 PM
To: "zbrafish from net.bio.net<mailto:zbrafish from net.bio.net>" <zbrafish from magpie.bio.indiana.edu<mailto:zbrafish from magpie.bio.indiana.edu>>
Subject: [Zbrafish] how to stop zebrafish heart beat

Hi,

We are experiencing difficulties in two-photon fluorescence imaging of live zebrafish embryos and larvae: although the skeletal muscle is relaxed by tricaine the heart is still beating and thus causes blurry images. So we would appreciate very much if someone could recommend a good way to stop fish heart during imaging (it would be ideal, if it is reversible so the fish could be revived after imaging).

Thanks very much.

Sincerely,

Weibin Zhou, Ph.D.

Assistant Professor
Division of Pediatric Nephrology
Department of Pediatrics and Commuinicable Diseases
University of Michigan Medical School

1150 W Medical Center Drive
8220E, Medical Science Research Building 3
Ann Arbor, MI 48109-0640

Phone: +1(734)647-9429(office); +1(734)615-7896 (lab)
Fax: +1(734)615-1386
Email: weibinz from med.umich.edu<mailto:weibinz from med.umich.edu>


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