Has anyone encountered low 260/230 readings (<0.2) using the Trizol
RNA extraction. We're getting okay yields and great purity using 5
embryos (24hpf-72hpf)/sample, but are having problems with what we
assume is contamination.
We have already tried a number of things: bought all our chemicals
new, repeat chloroform extraction 2 times, do EtOH cleaning step 3
times. We homogenize manually with pestles.
Thanks for any advice.
