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[Zbrafish] ZF-MODELS knock-out service

zfinadmn knight at uoneuro.uoregon.edu
Fri Mar 31 11:58:34 EST 2006


The ZF-MODELS consortium would like to draw attention to its public
and free knock-out service for zebrafish, based on the resequencing  of
mutagenized fish (TILLING). Up to 60 knock-outs will be performed  for
researchers outside the consortium, whose requests are considered  on
the basis of their scientific merit. The workload is distributed
between the laboratories of Sanger Institute (D. Stemple), Hubrecht
Laboratory (E. Cuppen), and TU Dresden (M. Brand). To request a
zebrafish KO mutant, please send a brief (approx. one page) summary  to
Dr. Edwin Cuppen <mailto:ecuppen at niob.knaw.nl>. This summary  should
contain the following information:

Information on the gene to be knocked out: Name of the gene and
Ensembl ID. Please note that the ZF-MODELS consortium will only  accept
requests for KOs based on Ensembl IDs. The requester should  also check
the Ensembl annotation. In case the annotation is  incorrect, the
requester has to make sure that it is changed. The ZF- MODELS
consortium will not screen for a gene as long as the  annotation is not
correct.
Scientific relevance: Explain why is it important to knock this gene
out.
Zebrafish background of the lab requesting the KO mutant: Explain how
any work on the KO mutant provided would be embedded in an  environment
in your lab where people can actually work with zebrafish  mutants.

The summaries received by Dr. Edwin Cuppen form the basis for
prioritized lists drafted up by the ZF-MODELS Executive Committee on  a
regular basis.

Once a request has been accepted, you will be asked to design an
amplicon(s) for your region(s) of interest through the LIMSTILL  online
tool <http://limstill.niob.knaw.nl/>. Oligos will be ordered  and
tested by the ZF-MODELS consortium. The progress of requests can  be
tracked through the internal knock-out website of Hubrecht  Laboratory
(login required).

Information on the knock-out mutants generated by the ZF-MODELS
consortium is made publicly available through the knock-out website
<http://www.niob.knaw.nl/researchpages/cuppen/zfmodels/> of Hubrecht
Laboratory, immediately after a knock-out is generated. Knock-out  fish
are immediately delivered to the requesters.

"ZF-MODELS - Zebrafish Models for Human Development and Disease" is  an
Integrated Project funded by the Sixth Framework Programme of the
European Commission. For further information please see the project
website <http://www.zf-models.org>.



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