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Fish Embryo Extract

Sapphire sgrutsis at WESTMONT.EDU
Wed Jul 10 18:50:55 EST 1996

Hello Newsgroup Readers-

I have a question regarding the preparation of Fish Embryo Extract.  The 
Zebrafish Book says to use 3 day old embryos after removing them from 
their chorions, however we have found that our fish have already hatched 
out of their chorions by the third day. Is it important that the fish be 
three days into development before being used for the extract? or  Is it 
more important to remove the embryos from their chorions just before the 

Responses should be sent to "sgrutsis at westmont.edu".  Thanks so much 
for your help!  Sophia

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