[Virology] RE: particle to pfu ratio

[Dunowska, Magda]

Doria,
If you have a pure virus prep, maybe you could titrate it (to get PFU/mL value), and then extract viral DNA and estimate the number of viral particles based on the amount of viral DNA present, knowing that parvoviruses have a single-stranded DNA genome about 5.2 kbp in size? Just a thought...

Magda



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Message: 1
Date: Wed, 7 May 2008 12:26:10 -0400
From: "Bowers, Doria" <dbowers from unf.edu>
Subject: [Virology] particle to pfu ratios
To: <virology from magpie.bio.indiana.edu>
Message-ID:
        <783CE756031AB141B53257D89039DD75075EFFA3 from DIAMOND.unfcsd.unf.edu>
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We are wondering what the particle:PFU ratio is for porcine parvovirus.



Any ideas how to acquire this statistic?



Doria Bowers











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