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[Virology] RE: particle to pfu ratio

Dunowska, Magda via virology%40net.bio.net (by M.Dunowska from massey.ac.nz)
Fri May 9 18:14:16 EST 2008

If you have a pure virus prep, maybe you could titrate it (to get PFU/mL value), and then extract viral DNA and estimate the number of viral particles based on the amount of viral DNA present, knowing that parvoviruses have a single-stranded DNA genome about 5.2 kbp in size? Just a thought...



Message: 1
Date: Wed, 7 May 2008 12:26:10 -0400
From: "Bowers, Doria" <dbowers from unf.edu>
Subject: [Virology] particle to pfu ratios
To: <virology from magpie.bio.indiana.edu>
        <783CE756031AB141B53257D89039DD75075EFFA3 from DIAMOND.unfcsd.unf.edu>
Content-Type: text/plain;       charset="US-ASCII"

We are wondering what the particle:PFU ratio is for porcine parvovirus.

Any ideas how to acquire this statistic?

Doria Bowers


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