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[Virology] Cervical Swabs for HPV PCR

Sümeyra Alkýþ via virology%40net.bio.net (by sumeyrakocturk from yahoo.com)
Wed Jul 30 03:11:49 EST 2008


Hello,I would like to hear from anyone who is actually doing PCR for Human
Papillomaviruses on DNA from cells obtained by cervical swabs, cytobrush,
cervical lavage etc.  Basically, what works best for you?  For example,
which do you recommend.. brush or swab or?,is the swab, brush etc. left dry
in collection tube?, in a transport media?, at what temperature?  How best
to separate cells?  extraction procedure? and so on.  
Many thanx for any good info.
Dr.Sumeyra kocturk from Turkey



      


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