I use seaplaque agarose from FMC for overlay for BHV-1 plaque assay.
You can microwave the agarose(which is sufficient) and mix with 2x DMEM or
similar medium with anitbiotic. I have adapted the protocol from :
You can also try this web site:
And there is a software also available for the plaque assay @
Gopinath Raju Seetharaman
Post-Doctoral Research Fellow,
University of Nebraska-Lincoln,
Dept. of Veterinary and Biomedical Sciences,
East Campus Loop and Fair street,
>From: "Frank M. Mattes" <mattes at rfhsm.ac.uk>
>To: bionet-virology at net.bio.net>Subject: plaque assay protocoll ?
>Date: Thu, 3 May 2001 14:19:35 +0100 (BST)
>>Dear newsgroup reader,
>>does anybody know a good protocoll to do a plaque assay for cmv ?.
>I know you need methylcellulose, wich is critical to avoid the spread of
>virus within the media.
>I'm wondering also if these clellulose media is also pre-mixed available
>from a commerical source.
>>Many thanks for your help
>Dept. of Virology
Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com.