I am currently trying to quantitate reverse transcriptase activity in
supernatents of cells which produce SNV particles. While I have obtained a
kit from Boerhinger Mannheim for the detection of RT activity, it fails to
work for SNV. I am told that it may be due to SNV RT being a
Manganese-dependent enzyme opposed to a Magnesium-dependent enzyme. While
this may be true, I would like to know if anyone has ever measured RT activity
Thank for any help anyone might have.
mpandori at netway.com