I am a longtime virologist but only recently HIV-doc.
I have been growing HIV in my lab but have had a lot of trouble
culturing up from frozen plasma (as opposed to co-culture with PBMC's).
I have been looking around for high sensitivity methods but don't want
to spend a lot of effort re-inventing the wheel. Right now I am using
the standard ACTG methods but I would be very interested in any
improvements others have worked out or any good references people know
Division of Infectious Diseases
Beth Israel Deaconess Medical Center
Harvard School of Medicine
robin at louis.bidmc.harvard.edu