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Increasing virus titer

A.J.Cann nna at leicester.ac.uk
Tue Oct 21 10:32:08 EST 1997


In article <62cbbr$h6r at net.bio.net>, Jeffrey Bunch <jbunch at earthlink.net> wrote:

>Enhancing virus attachment is of interest to me as I require the highest
>infectious units I can obtain for my work.  I work with a variety of
>animal and human viruses.  For example I can obtain 10 logs of EMC in
>L929 cells but then I have difficulty even obtaining 7 logs of Porcine
>Parvovirus in PK-13 cells. I am in great need of improving the culturing
>conditions for PPV.  Besides adding polybrene and lowering the pH (for
>enhancing virus attachment) do you have any suggestions?

The pH & polybrene suggestions are quite specific to certain viruses, not
general methods. For most viruses, the most practical methods is
concentration of a virus containing supernatent. You MIGHT be able to get
way with using a membrane concentrator such as one of the Amicon devices,
but since this concentrates a lot of crud as well as virions, it is usually
necessary to use centrifugation (either banding or pelleting the virus).
The precise protocol to use depends on the virus you are working with.

-- 
Dr Alan J. Cann  PhD, Department of Microbiology & Immunology,
University of Leicester, UK.






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