Hi everybody,
today I have a really serious question t everyone beeing familiar with
molecular biology.
Here my problem:
Since more than a century now, I am trying to clone a PCR fragment, which
I designed XhoI-XhoI, into an Xho-NheI. However, since today I have not
been able to get a single positive clone. Why?
Does anyone has valuable suggestions, I really would appreciate it.
Thanks in advance.
