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sedimentation at 1g...

Andrew Albright albright_a at a1.mscf.upenn.edu
Thu Mar 28 08:01:32 EST 1996


In article <m0u1s1w-0005UHC at uctmail2.uct.ac.za>, ed at molbiol.uct.ac.za wrote:

> I have just seen the results of centrifuging a TMV-U2 and a BSMV 
> preparation at 1g for 26 yrs and 11 yrs, respectively...the viruses 
> SEDIMENTED!!  There was a beautiful gradient in both cases, of 
> uncontaminated and not-obviously-aggregated virus, from crystal clear 
> ant the top of a 100 ml medical flat bottle, to thick and milky at 
> the bottom - with most of the stuff at the bottom.  I am keeping one 
> BSMV bottle untouched; we shook up the rest in the interests of 
> scientific inquiry (we were clearing out a coldroom in preparation 
> for Barbara von Wechmar's retirement at the end of the year).  BMV 
> preparations left the same length of time (spherical, 30 nm, +/-100S) 
> are uniformly suspended still - but rod-shaped chaps of 150-300 nm 
> and +/-200S sediment beautifully.
> 
Ed,
This sounds very interesting. In the interest of scientific inquiry I
would request that you send me some of the virus so that I can repeat your
findings.  I would understand if you would want to hold of on sending me
some until you publish this.  
Thanks,
Andrew

-- 
Andrew Albright(albright_a at a1.mscf.upenn.edu)



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