I have just seen the results of centrifuging a TMV-U2 and a BSMV
preparation at 1g for 26 yrs and 11 yrs, respectively...the viruses
SEDIMENTED!! There was a beautiful gradient in both cases, of
uncontaminated and not-obviously-aggregated virus, from crystal clear
ant the top of a 100 ml medical flat bottle, to thick and milky at
the bottom - with most of the stuff at the bottom. I am keeping one
BSMV bottle untouched; we shook up the rest in the interests of
scientific inquiry (we were clearing out a coldroom in preparation
for Barbara von Wechmar's retirement at the end of the year). BMV
preparations left the same length of time (spherical, 30 nm, +/-100S)
are uniformly suspended still - but rod-shaped chaps of 150-300 nm
and +/-200S sediment beautifully.
A bit long to wait to concentrate virus tho...but so ELEGANT.
Ed Rybicki, PhD
Dept Microbiology | ed at molbiol.uct.ac.za
University of Cape Town | phone: x27-21-650-3265
Private Bag, Rondebosch | fax: x27-21-650 4023
7700, South Africa |
WWW URL: http://www.uct.ac.za/microbiology/ed.html
"And then one day you find, ten years have got behind you"