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HELP: Why 50bp marker is a smear in denatured gel?

Brian Foley brianf at med.uvm.edu
Thu Jun 27 16:28:24 EST 1996

Bixing Huang (huangbx at DEAKIN.EDU.AU) wrote:
: I am trying to get denatured gel  (5M urea) running in my lab. The problem I
: am encountered is the 50bp DNA marker is a smear after silver stained.

	Possible causes:
1) loading too much 50 bp marker per lane
2) not rinsing urea from well before loading marker
3) running gel at wrong temperature
4) 50 bp marker not in proper loading buffer

*  Brian Foley          *  btf at t10.lanl.gov                        *
*  HIV Database, LANL   *  http://hiv-web.lanl.gov/index.html      *
*  Los Alamos, NM 87545 *  http://hiv-web.lanl.gov/~btf/home.html  *

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