Flu isn't one of the viruses I've worked with, but our lab is going to be
playing with some for a while. We received a large volume of frozen
virus, which we tossed into our -80 oC freezer while we looked dubiously
at each other. We need to thaw and aliquot this. Can anyone tell me:
-how much reduction in titre we can expect from the freeze-thaw?
-how to accurately titrate the stocks, if we need to repeat the titre?
-anything else a novice flu person should know?
We're just going to be using this as targets in cytotoxic T cell assays,
so we don't need to get fancy with it.
Ian York (iayork at panix.com) <http://www.panix.com/~iayork/>
"-but as he was a York, I am rather inclined to suppose him a
very respectable Man." -Jane Austen, The History of England