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CMV promoter & human macrophages

Laurence Tiley lst21 at cam.ac.uk
Mon Jul 1 03:59:06 EST 1996

Hi Beverley.

Yes the CMVie promoter works in human macrophages. Most studies have 
used promyelocytic cell lines to study this, but there are a few studies that 
have used freshly isolated macrophages. Your "someone" may be confusing 
macrophages with monocytes, that contain a repressor of CMVie activity. 
Monocytes can be infected, but need to differentiate before the promoter 
becomes fully active. The repressor binds to the 21bp repeat, so I guess that 
if this site is mutated, you could get a CMV promoter that works in both.

Hope this was useful.


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