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RNA extraction

Lee R. Martin LRM at zeus.ahabs.wisc.edu
Tue Jan 30 13:32:57 EST 1996


I too work on picornaviruses. We have a lab protocol for extracting vRNA
from infected cell lysates: 

acid phenol (selects against DNA)
TE-equilibrated phenol
phenol/chloroform
chloroform
EtOH ppt. for 60'


The resulatant vRNA works well for RT-PCR. Hope this is of some help and
that you don't mind phenol (I wasn't sure)

-- 
Lee R. Martin
LRM at zeus.ahabs.wisc.edu



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