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cDNA purification

m.j.carter M.Carter at surrey.ac.uk
Tue Jan 9 12:09:33 EST 1996


hi,

Has anyone used these wizard preps to purify newly made ds cDNA. At this
stage the cDNA consists of 1 continuous strand made by the RT and a
gapped/discontinuous strand made by the DNA pol priming on the RNAse
H-nicked RNA. Does this sort of structure bind to the resin? 

Cheers

Mike




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