Marry christmas Netters
I am constructing a cDNA library from 100ng of viral genomic
RNAs (Broad bean wilt virus ; BBWV) with two types of cDNA synthesis
kit (produced by Phamacia, Stratagene). I use random hexamer and Oligo
(dt) as primers, because genomic structures of BBWV are not reported
yet, specific primers are not available.
But even first-stranded cDNA was not synthesized from viral RNA
I am looking for ways and means to synthesize the first cDNA from
viral RNA templates.
I think that secondary structures of viral RNAs inhibit the
extension of the primers.
Any suggestions in this regard will be appreciated.
email:unglee at life.postech.ac.kr