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Postdoctoral Position

Marleen Lippert MSL at plantpath.wisc.edu
Sat Aug 3 13:16:55 EST 1996

A VIROLOGY POSTDOCTORAL POSITION will be available in September of
1996 at the Department of Plant Pathology, University of Wisconsin-
Madison.  The genus Tospovirus contains the plant-infecting members of
the family Bunyaviridae, a large group of enveloped viruses with
tripartite gnomes of negative and ambisense RNA (Reviewed by German et
al., 1992, Ann. Rev. Phytopathol. 30:315-348).  Negative-sense RNA
viruses encapsidate a virus encoded RNA-dependent RNA polymerase
(RdRp) which initiates the intracellular replication cycle by
transcribing mRNAs from the viral genomic RNAs.  We have recently
demonstrated the presence of an RdRp activity associated with
detergent-disrupted virions (Adkins et al., 1995, Virology 207, 308-
311) and nucleocapsids of tomato spotted wilt virus (TSWV, the type
member of the Tospovirus genus).  Stimulation of TSWV RdRp activity by
capped RNAs suggests that cap snatching is supported by this system. 
Preliminary results predict that helicase and nuclease activities are
also present.  Research will address specific experimental goals to
characterize RdRp, helicase, nuclease and cap snatching activities in
the in vitro RSWV RNA synthesis system and to identify the protein(s)
mediating these activities.  Longer term goals are to characterize a
subcellular fraction from TSWV-infected plants capable of supporting
TSWV RNA synthesis and test the replication competence of in vitro
transcripts to identify critical RNA sequences.  Applicants should
have experience in molecular virology but prior work with plants is
not required.  To apply send a cover letter describing research
experience and interests, a curriculum vitae and the names of three
references to:  Dr. Thomas German, University of Wisconsin-Madison,
Department of Plant Pathology, 1630 Linden Drive, Madison, Wisconsin
53706.  FAX:  608  263-2626;  E-Mail:  tlg at plantpath.wisc.edu.  The
University of Wisconsin is an Equal Opportunity Employer. 
Thomas Pfitzner
Moderator bionet.virology
University of Saarland, Homburg/Saar, Germany

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