UNIVERSITY OF QUEENSLAND and
THE SIR ALBERT SAKZEWSKI VIRUS RESEARCH CENTRE
Ph.D. SCHOLARSHIPS IN MOLECULAR VIROLOGY /
For 3 years commencing January, 1996
Two scholarships are available for students with a B.Sc. Hons.
1 or 2A degree obtained with training plus research experience in the
area of molecular biology, or of cell biology and ultrastructural
analysis. Current value of the Scholarship is $14,961 per annum.
Students will be based at the Sir Albert Sakzewski Virus Research
Centre (SASVRC at the Herston Campus, with Professor E Westaway and Dr
A Khromykh) or the Centre for Microscopy and Microanalysis (St Lucia
campus, with Dr M Jones) for an integrated project funded by NHMRC.
The research aims to define the sites of flavivirus replication and
the roles of the seven nonstructural (ns) proteins encoded by Kunjin
virus (related to Murray Valley encephalitis, yellow fever and
dengue). Kunjin virus has been a key laboratory model for the
molecular biology of flaviviruses for many years.
Each of the Kunjin ns genes has been expressed from cDNA in vivo and
in vitro, and specific antibodies prepared against all the purified ns
proteins. Infectious Kunjin RNA and self replicating subgenomic RNA
or replicons are transcribed as required from the stable cDNA
constructs at SASVRC. The latest techniques for cryo-electron
microscopy and immunogold labelling of infected cells are already
established by Dr Jones at St Lucia. One student will be involved in
the molecular biology of flavivirus replication, preparing mutated
viruses, replicons and specific ns genes via cDNA changes. Expression
and biochemical analyses of the effects of specific mutation on virus
replication will be analysed. The other student will use wild type
virus and the genetic constructs introduced into cells for analyses of
induced changes in ultrastructure and definition of the location of
individual ns proteins in replication complexes. Facilities and
equipment are excellent at both locations.
This integrated project is a novel joint approach to unravelling the
events of flavivirus replication, and the results will find
application to other viruses of medical and veterinary importance with
similar replication strategies. Such studies could lead to the design
of antiviral drugs or vaccines.
Interested students please contact Professor Westaway (phone (617)
3253 1400 or fax (617) 3253 1401) or Dr. Khromykh (phone (617) 3253
1569, Email A.Khromyk at mailbox.uq.oz.au) or Dr Jones (phone 3365 3249
or fax 3365 2199, Email: m.jones at mailbox.uq.oz.au) for further
The information is also available on
Dr. Alexander Khromykh
Sir Albert Sakzewski Virus Research Centre
The Royal Chidren's Hospital
Herston Rd, Herston, Brisbane, Qld,
Phone: 617 3253 1569
Fax: 617 3253 1401
Email: A.Khromykh at mailbox.uq.oz.au