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Westerns on uninfected H9 cells

Jonas.Blomberg at alinks.se Jonas.Blomberg at alinks.se
Tue Oct 24 17:50:05 EST 1995

We ran such Westerns routinely together with HIV protein 
containing ones for about 5 years. We found some anticellular 
reactions in the early HIV blots which could be sorted out with 
H9 cells. Later, the HIV blots became much better, i.e. both more 
sensitive and more specific. The comparison with H9 strips ceased 
to be meaningful. We still have some in the freezer for a 
problematic false positive serum, but we did use them for 3 years 
now. The main H9 band in the early blots was around 55 Kd, with 
sera from patients with glomerulonephritis. We never identified 
it. Nowadays we do not see it in the HIV blots.


*************< Jonas.Blomberg at alinks.se >************
 Jonas Blomberg, M.D., Ph.D.
 Dept. Medical Microbiology (division Virology)
 University of Lund
 Solvegatan 23
 S-223 62 Lund, Sweden

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