In article <45h2lr$et2 at solaris.cc.vt.edu>, Bill Zabaronick <zab at vt.edu> wrote:
> I am interested in trying to purify released virions from the liquid
> media that surrounds my cells growing in a monolayer. Is there a
> good(effective) HPLC method? If so could I get it? If not, are there any
Most viruses are purified from culture medium by centrifugation. There is
usually a brief centrifugation of the supernatant fluid to remove cellular
debris followed by a gradient centrifugation through glycerol or sucrose.
Unless your talking about a very small amount of virus I don't see where
HPLC would save time or effort. If you know the identity of your virus
just check the literature. The M & M section of almost any paper should
have a virus purification scheme.
Steven A. Enkemann PhD.
I don't believe that curiosity killed the cat.
I think it just did the wrong experiment.