I degraded Bohle Iridovirus with NP40 and DMSO and spun the suspension
through a 10% sucrose cushion, collecting the supernatant and resultant
pellet.The pellet was reusupended in TNE buffer.I was wondering if
anyone could help me with the following:
- Would the NP40 and DMSO enter the gradient and become mixed with the
pellet requiring dialysis, or is this step unneccesary?
-I also degraded the virus with octylglucoside (OTC) and spun the
suspension through a 10% sucrose cushion containing OTC. Again, would the
pellet become mixed with the OTC requiring dialysis, or is this dialysis
I only have a small amount of each sample and do not want to waste any.
Department of Biomedical and Tropical Veterinary Sciences
James Cook University
Townsville, Qld., 4811
Tel: +61 077 814 631
Fax: +61 077 796 371