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M13 ssDNA stability

Dubear Kroening dubear at whitewater
Fri Mar 31 08:22:50 EST 1995


We are noticing degradation of M13 ssDNA when resuspended in water and 
heated at 90 degrees celcius for 10 minutes.  We see no degradation 
when just resuspended in water (without heating) or when resuspended 
in TE (with or without heating).  We were concerned that this may have 
something to do with magnesium in the prep.  Does anyone have any 
ideas at all?  Any comments or suggestions would be greatly 

Dubear Kroening
dubear at whitewater.chem.wisc.edu

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