Dermot Cox (dcox at medmicro.uct.ac.za) wrote:
> We are at present having problems transfecting primary cells derived from
> foetal bovine testes with plasmid DNA. We are using the DOTAP kit from
> Boehringer-Mannheim with little success. Has anyone had similar problems with
> this kit or could make suggestions of alternate methods.
I regularly use Calcium-Phosphate (protocol found in Current Protocols).
If you don't have access to that protocol, I'll post it for you.
Benoit_Hebert at iaf.uquebec.ca
Virology Research Center
Armand-Frappier Institute, Laval, Quebec