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Transfecting primary cell lines

Benoit_Hebert at IAF.UQUEBEC.CA Benoit_Hebert at IAF.UQUEBEC.CA
Wed Mar 1 21:01:00 EST 1995

Dermot Cox (dcox at medmicro.uct.ac.za) wrote:
> We are at present having problems transfecting primary cells derived from 
> foetal bovine testes with plasmid DNA.  We are using the DOTAP kit from 
> Boehringer-Mannheim with little success.  Has anyone had similar problems with 
> this kit or could make suggestions of alternate methods.

I regularly use Calcium-Phosphate (protocol found in Current Protocols).
If you don't have access to that protocol, I'll post it for you. 

Benoit Hebert

Benoit_Hebert at iaf.uquebec.ca

Virology Research Center
Armand-Frappier Institute, Laval, Quebec

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