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Transfecting primary cell lines

Dermot Cox dcox at medmicro.uct.ac.za
Wed Mar 1 15:52:31 EST 1995


We are at present having problems transfecting primary cells derived from 
foetal bovine testes with plasmid DNA.  We are using the DOTAP kit from 
Boehringer-Mannheim with little success.  Has anyone had similar problems with 
this kit or could make suggestions of alternate methods.

Thanks 
Dermot Cox
Dept. Med. Micro
UCT Medical School
Cape Town
South Africa



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