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polybrene question

Matthew Dougals Koff mkoff at coos.dartmouth.edu
Fri Jan 27 18:12:26 EST 1995

In article <3gbe7h$3d2 at hsc.usc.edu>, chale at hsc.usc.edu (Chuckles) wrote:

> zxy5 at po.CWRU.Edu (Zafer Yildirim) writes:
> >We are using recombinant retroviruses for gene transfer to some
> >mammalian cells. We include polybrene during the infection of 
> >these cells with the viruses. Can somebody tell
> >me how polybrene works to enhance infection?
> >Thanks in advance.
> The theory that I have heard is that polybrene coats membranes and thus
> the negative charges on the surface.  This allows membranes to come together
> more easily and facilitates viral fusion.  Unfortunately I don't have
any referances
> that support this, but you do see enhanced cell-cell fusion in the presence of
> polybrene which gives some support to this idea.  Hope this helps a little.
>                                         Charles Hale
>                                         USC Department of Biochem.
>                                         "No Pain, No Pain!!!"

I did a bunch of projects for my Master's thesis using Retroviral Vectors
and I found a reference that described basically what Charles Hale just
said.  I will look it up and get it to you. Don't have my Thesis on me
now. But will get back to you.

Dartmouth Medical School
See ya' on the slopes...

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