I am trying to express a protein which is supposed to be a
product of a 5406 bp DNA fragment. However the actual ORF is small and
the gene reads on the complementary strand.
I HAVE SOME QUESTIONS!
1- Maximum how many extra bases I can have before the ATG
codon of my "gene"?
2- It appears that I do not have a lot of desirable restriction
sites around the beginning of the ORF, how am I going to get rid of the
3- I am planning to use polyhedrin promoter based vectors. The
kits that the companies sell are very expensive (almost $500 for 5
transfections). Which one is the best choice? Is occ based selection too
Thanks for the help!