In article <hamel.819476001 at pollux>, hamel at cc.umanitoba.ca (Andre Hamel) wrote:
> Is anyone aware of any publication references regarding reconstruction of
> viruses or phage from recombinant bits?
>> For example, transfecting cells with various parts of phage genome and
> obtaining complete, viable phage (as result of recombinant in the host cell).
>> I did a Medline search but I suspect that the keywords that I had used
> were not adequate.
>> Many thanks,
>>>> ------------------------------------
> Andre Hamel email: hamel at cc.umanitoba.ca> (Scientist) tel: 204/945-7630 FAX: 204/945-8062
> Virology Laboratory, Animal Health Centre
> Manitoba Government Department of Agriculture
> 545 University Crescent, Winnipeg, Manitoba, CANADA R3T 5S6
Andre,
you might check 'Robertson, E., and E. Kieff. 1995. Reducing the
complexity of the transforming Epstein-Barr virus genome to 64 kilobase
pairs. J. Virol. 69:983-993' and previous papers by E. Kieff's group.
Similarly, pseudorabies virus can be reassembled by just transfecting
partially overlapping cosmid clones into cells.
Good luck searching,
Wolfgang
--
Forschungszentrum fuer Umwelt und Gesundheit
Institut fuer Klinische Molekularbiologie und Tumorgenetik
Marchioninistr. 25
D-81377 Muenchen
Germany
