> From: Alexander.Brozeit at arbi.informatik.uni-oldenburg.de (Alexander Brozeit)
> Subject: [Q] REM/SEM microscopy of viruses
> As a physicist I was asked if there is a possibility to detect
> viruses by means of REM/SEM microscopy.
> In principal this technique is feasible, I know, the question
> addresses the way of specimen preparation: small eruptions of
> the skin contain an almost clear liquid which is directly applied
> to a microscope cover plate. After desiccating, the specimen is
> sputtered with approx. 3 to 5 nanometer Gold.
>> Is it likely to find (if prominent) a virus by using this method ?
> If not, which technique is more likely to yield success ?
Plant virologists simply take some clarified sap or a drop of clear
sap expressed from a leaf/stem and put onto a grid and negative
stain: and I heard from a rotavirologist recently that he was taking
"clarified faeces" and doing exactly the same thing. Strikes me as
being a departure back to the 1940s to coat them by sputtering.
| Ed Rybicki, PhD | ed at molbiol.uct.ac.za |
| Dept Microbiology | University of Cape Town |
| Private Bag, Rondebosch | 7700, South Africa |
| fax: x27-21-650 4023 | phone: x27-21-650-3265 |
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