Rocco,
>The problem:
>The human sera appears to be picking up HeLa cellular proteins in
>general. This has been observed with plain HeLa cells, as well,
>when they are used as negative controls.
The HeLa cell line was derived from an HPV-cervial carcinoma, I would
suspect that pooled human serum would have anti-HPV antibodies. Is it
possible that your background noise correlates with HPV genes expressed
in the HeLa cells (E6, E7, others?).
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