In article <3vr01n$8sb at news.orst.edu> Steven Poet <poets at ccmail.orst.edu> writes:
>That seems like a pretty high dilution to me. Are you sure your decimal
>points are all in the right place? I remember somewhere in the depths
>of my memory that 10^17 poliovirus particles will fill a ping pong ball.
>So I don't think 10^23 virus particles can be suspended in 1 ml of
>cell culture medium. I understand the difference between TCID50 and
>absolute particle numbers, I'm just thinking intuitively.
>>krjk1 at stirling.ac.uk (Mr K R John Kollanoor) wrote:
>>>> Is there a theoratical maximum for the number of virus progeny a cell
>> culture supernatant can have?
>> Is there any possibility of a titer of 10^-23(TCID50/ml) in any of the virus
>> infected cell culture supernatant ?
>> I would appreciate if someone could answer these questions.
>>>I recall poliovirus stocks with titers around 10^10 per ml or 10^12 particles
per ml. 10^23 seems much too high. Look at the size of a lentivirus:
120 nM (120 x 10^9 M) in diameter
8 x 10^6 particles along a one meter line
5 x 10^14 particles packed into a cubic centimeter (one ml)
5 x 10^20 particles packed into a cubic meter
Greg Tobin, Ph.D. tobin at lcms-1.ncifcrf.gov