Hi, I can't give you any information myself, but if you contact Olov
Grankvist and Ake Gustafsson at the Department of Virology, Umea
University, Umea, Sweden, they'll have all you need and more about HIV
primers used in PCR. The email adress to them
is:"Ake.Gustafsson at climi.umu.se". Just show them this message from me. By
the way, my name isn't Nigel McMillan, I'm Magnus Evander and I'm using
this emailadress only because this computer is connected to the internet.
My own emailadress is: "mevander at gpo.pa.uq.oz.au" Good luck,
tmiller at newssun.med.miami.edu (Todd Miller - Pharmacology) wrote:
> I've noticed that the PCR, applied to HIV, almost always
>uses the SK primers that amplify part of the gag sequence. Does
>anyone know of studies that compared the results of this primer
>pair to other primer pairs also specific for HIV, but in other
>parts of the genome? Are there factors that would limit the
>degree of correlation between the results of different primer
>pairs? How close would you expect different primer pairs to be
>as far as providing an answer to 1)infected or not infected and
>2)quantitative measurements of viral load?
>> Thanks for any help. This question was too tough to
>extract out of the literature, so I'm hoping someone has come
>across this, perhaps even WRT other viruses.