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Maximum possible virus titers

g1687jkarh at umbsky.cc.umb.edu g1687jkarh at umbsky.cc.umb.edu
Thu Aug 3 13:07:54 EST 1995

In Article <3vouor$cqb at lorne.stir.ac.uk>
krjk1 at stirling.ac.uk (Mr K R John Kollanoor) writes:
>Is there a theoratical maximum for the number of virus progeny a cell 
>culture supernatant can have? 

     Yes. Using cgs units (except where specified, or where no units)

            6.02 * 10^23 * density * fraction of weight which is virus
                       weight of a virion in daltons

     How much virus (in mass percent or other stated units) could there be
in something which is a supernatant, rather than a pellet or pellicle?        

>Is there any possibility of a titer of 10^-23(TCID50/ml) in any of the virus 
>infected cell culture supernatant. 

     Yes. Take a sample of nasal secretions from someone with the flu.
Put it into a liter of water and autoclave it for a few hours. Though, it 
would be difficult to measure, the titer would likely be around 10^-23 per ml. 

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