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cDNA PCR on RNA virus

Patrick O'Neil patrick at corona
Thu Apr 6 13:27:08 EST 1995



On 6 Apr 1995, Ed Rybicki wrote:

> 
> Any hints on good cDNA PCR for longer products?
> 

Have you tried nested primers simultaneously with normal end primers?  
Longer elongation time?  Mg++ titrations?  

Forgive me if these are considered very basic but since there is no 
mention of what you have tried thus far...

Patrick



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