I know there was some discussion on this...we want to PCR about 2.2
kb of a cucumber mosaic virus RNA, and although we can do smaller
pieces no prob, the bigger eludes us.
Any hints on good cDNA PCR for longer products?
Which enzymes, what conditions?
Ta in advance,
| Ed Rybicki, PhD | ed at molbiol.uct.ac.za |
| Dept Microbiology | University of Cape Town |
| Private Bag, Rondebosch | 7700, South Africa |
| fax: x27-21-650 4023 | phone: x27-21-650-3265 |
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