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cDNA PCR on RNA virus

Ed Rybicki ED at molbiol.uct.ac.za
Thu Apr 6 05:16:12 EST 1995

I know there was some discussion on this...we want to PCR about 2.2 
kb of a cucumber mosaic virus RNA, and although we can do smaller 
pieces no prob, the bigger eludes us.

Any hints on good cDNA PCR for longer products?

Which enzymes, what conditions?

Ta in advance,
 |     Ed Rybicki, PhD      |  ed at molbiol.uct.ac.za   |
 |    Dept Microbiology     | University of Cape Town |
 | Private Bag, Rondebosch  |   7700, South Africa    |
 |   fax: x27-21-650 4023   | phone: x27-21-650-3265  |
 ------WWW URL: http://www.uct.ac.za/microbiology------
   "And the man in the suit has just bought a new car
      From the profit he's made on your dreams..."

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