I am analyzing polyprotein processing by in vitro translation of large
transcripts encoding polypeptides in excess of 200-kDa. Presently I am
capable of translating peptides of a maximal size of 120 k-Da in a wheat
germ or rabbit reticulocyte lysate. Does anyone have infomation or tips
that would help me to translate viral polypeptides of 200-400-kDa in either
of these extracts.