In article <2je8qd$gkc at charm.magnus.acs.ohio-state.edu> tchang at magnus.acs.ohio-state.edu (Tien-Hsien Chang) writes:
> I have attempted to retrieve the complete sequence information of
>bacteriophages Q-beta and R17 without success. Does anyone know whether these
>two phage genomes have been completely sequenced?
>> I am mainly interested in the complete sequence information of the COAT
>PROTEIN genes in both phages. I am also searching for sources where I can
>obtain these "cloned" genes.
>> Your helps are much appreciated.
>>> Tien-Hsien Chang, Ph.D.
> Assistant Professor of Molecular Genetics
> The Ohio State University
> Columbus, OH 43210
> (614)-292-4466 [fax]
>tchang at magnus.acs.ohio-state.edu
DNA WorkBench found the following.
DNA(At 962 of 962)% searches
Size Number Command
149 1 text q-beta (in gball gbnew)
147 2 text r17 (in gball gbnew)
666 3 text coat (in gball gbnew)
DNA(At 962 of 962)% intersection 1 3
DNA(At 5 of 5)% headers 1-5
1 LEQBET first half of the phage Q-beta gene for coat protein.
2 PQBCP5 bacteriophage qbeta coat protein cistron first half.
3 M24876 Figure 3. RNA sequence around the beginning of the replica
4 ECOA1COAT Escherichia coli RNA bacteriophage Q-beta coat protein
5 ECQB120 E. coli/Q-beta phage recombinant RNA RQ120
DNA(At 5 of 5)% intersection 2 3
DNA(At 7 of 7)% headers 1-7
1 MS2CG Bacteriophage MS2, complete genome.
2 PODOT7 Genome of bacteriophage T7.
3 R17APROT bacteriophage r17 a protein initiation site.
4 R17COATP bacteriophage r17 coat protein initiation site.
5 R17CP5 Bacteriophage R17 coat protein gene, 5' end.
6 R17CPRAA Bacteriophage R17 coat protein RNA fragment.
7 R17RNASYN bacteriophage r17 rna synthetase initiation site.
The complete sequence could then be obtained by typing the number of the
sequence of interest.
(DNA WorkBench is available from cbil.humgen.upenn.edu:/pub/dnaworkbench).
Departments of Genetics and Computer and Information Science
Computational Biology and Informatics Laboratory, Human Genome Project
University of Pennsylvania
tisdall at cbil.humgen.upenn.edu