IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Expression of viral antigens

H6097Ben%HUELLA.earn at uk.ac.earn-relay H6097Ben%HUELLA.earn at uk.ac.earn-relay
Mon Oct 11 03:27:02 EST 1993


Subject: RE: Expression of viral antigens
 
: We have a postdoc starting in this lab soon, to work on expression
: of viral proteins for use as antigens in diagnostic tests (ultimately!).
: Can anyone offer advice on what system we should go for?
 
Dear Jon,
E. coli or even baculovirus will not produce authentically processed
mammalian viral proteins. The best systems of the well studied ones are
vaccinia and adenovirus. However, you may consider also the use of bovine
herpesvirus 1. It is not human pathogen like many viruses used as expression
vector, e.g., HSV-1, VZV, human adenovirus, rhinovirus, vaccinia. It is
neither a lethal animal pathogen as is any pseudorabies (Aujeszky's
disease virus or suid herpesvirus 1) based expression vector. LacZ reporter
gene inserted into its genome can be easily replaced by the desired new gene,
and color screening can be used to identify the positive mutants even if
no antibody is available against your protein. This vector neither require
any special cell line (e.g., a TK negative one). "Of course" it is replication
competent, so there is no need for helper virus. Let me know if you need further
information.
"Happy choosing"!
 
Balazs
________________________________________________________________________
                Balazs Harrach
                Veterinary Medical Research Institute,
                Hungarian Academy of Sciences
         (__)   H-1581 Budapest, P.O.B. 18.       _--------_
         (oo)   Hungary                          |__________|   BIG
  /-------\/    Tel.: +36 1-252-2455              XXXXXXXXXX    MAC
 / | 007 ||     Fax:  +36 1-252-1069              __________
*  ||----||     E-mail: h6098har at huella.bitnet   |_        _|
   ~~    ~~                                        --------
Cow licenced to kill           Enemy Cow after having met previous cow
________________________________________________________________________
 



More information about the Virology mailing list

Send comments to us at biosci-help [At] net.bio.net