I am using earthworms (E. foetida) to examine availability and
toxicity of soils with various oil fractions. In preliminary 7-day
assays I have not found mortality even at rather high concentrations.
However, I have seen what appear to be some dark markings (spots
or pores) on the clitella of some worms. The apperance of these
spots was not dose-dependent. If someone has similar observations
I'd appreciate some coments on what this could be.
My preliminary assay was to check mortality alone but a more
refined endpoint may be appropriate. Does anyone have
suggestions? I am considering weight changes or burrowing time
into a clean soil.
Thanks
Antonio Quiñones
