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Thiols in catalase and SOD

Chris Evelo C.Evelo at Farmaco.UniMaas.nl
Thu Apr 23 10:36:39 EST 1998

Hi Chuck 

Thank you very much! 

I should have added that I am looking for human erythrocyte catalase and

Our specific interests are (as mentioned before) thiols important for
activity and thiols located on the outside (able to crosslink) I am not
sure whether that can be derived from the sequences in the databases.

                Chris Evelo

Chuck Miller wrote:
> Hi Chris and others,
> There are many SOD and catalase sequences entered in the electronic
> databases -e.g. SwissProt, Genbank, etc., which can be searched, retrieved,
> and examined for location of Cys residues. Here are a few structural
> references.
> Prog Biophys Mol Biol 1997;67(2-3):217-252
> Structural chemistry and biology of manganese metalloenzymes.
> Christianson DW
> Department of Chemistry, University of Pennsylvania 19104-6323, USA
> Protein Sci 1997 Feb;6(2):481-483
> Crystallization and preliminary structural analysis of catalase A from
> Saccharomyces cerevisiae.
> Berthet S, Nykyri L, Bravo J, Mate MJ, Berthet-Colominas C, Alzari PM,
> Koller F, Fita I
> Protein Sci 1998 Mar;7(3):545-555
> Subunit asymmetry in the three-dimensional structure of a human
> CuZnSOD mutant found in familial amyotrophic lateral sclerosis.
> Hart PJ, Liu H, Pellegrini M, Nersissian AM, Gralla EB, Valentine JS,
> Eisenberg D
> UCLA-DOE Laboratory of Structural Biology and Molecular Medicine,
> University of California, Los Angeles  90095, USA.
> Biochemistry 1998 Apr 7;37(14):4731-4739
> Probing the active site of human manganese superoxide dismutase:
> the role of glutamine 143. Hsieh Y, Guan Y, Tu C, Bratt PJ, Angerhofer A,
> Lepock JR, Hickey MJ, Tainer JA, Nick HS, Silverman DN Departments of
> Pharmacology and Therapeutics, Biochemistry and Molecular Biology, and
> Chemistry, University of Florida, Gainesville, Florida 32610, Department of
> Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines
> R.
> J Mol Biol 1997 Dec 5;274(3):408-420
> Unique structural features of the monomeric Cu,Zn superoxide
> dismutase from Escherichia coli, revealed by X-ray crystallography.
> Pesce A, Capasso C, Battistoni A, Folcarelli S, Rotilio G, Desideri A,
> Bolognesi M
> Dipartimento di Fisica and INFM, Universita' di Genova, Largo Rosanna
> Benzi, 10, 16132 Genova, Italy.
> Am J Respir Cell Mol Biol 1997 Oct;17(4):393-403
> Mouse extracellular superoxide dismutase: primary structure,
> tissue-specific gene expression, chromosomal localization, and lung
> in situ hybridization. Folz RJ, Guan J, Seldin MF, Oury TD, Enghild JJ, Crapo JD
> Department of Medicine, Duke University Medical Center, Durham, North
> Carolina 27710, USA.
> Best of results,
> Chuck
> Dr. Charles A. Miller, III,   rellim at mailhost.tcs.tulane.edu
> Bionet.toxicology newsgroup: http://www.bio.net/hypermail/TOXICOLOGY
> Dept. Environmental Health Sciences, SL29
> Tulane-Xavier Center for Bioenvironmental Research and
> Tulane Univ. School of Public Health and Tropical Medicine
> 1430 Tulane Ave.
> New Orleans, LA 70112
> (504)585-6942, fax (504)584-1726

 Dr.Ir. Chris T.A. Evelo PhD
 Department of Pharmacology & Toxicology; Universiteit Maastricht
 P.O. Box 616; NL-6200 MD Maastricht; The Netherlands
 tel: +31-43-3881231 +31-43-3881417;  fax: +31-43-3670940
 email: C.Evelo at Farmaco.UniMaas.nl

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