IUBio

[Staden] prefinish for automated primer suggestion

deKesi squig at web.de
Thu Sep 28 15:20:43 EST 2006


Hello,

I would like to use prefinish in an automated way to suggest primers for 
assembly finishing.
Due to a lack of documentation I'm not sure if this is possible at all.
Can I use prefinish from the console to suggest primers for an gap4 - 
assembly?
Where can I find a more detailed documentation about this tool and his 
features?
If prefinish isen't able to perform these task I would consider to use 
autofinish. But I'm not sure how to convert the gap4 database into .ace 
format for use by autofinish. Any hints on these?

With kind regards,

deKesi



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