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Primer design in Gap4

Paul Shinn pshinn at mail2.sas.upenn.edu
Sun Mar 28 12:41:09 EST 1999

   When designing primers within the Contig Editor, a score value is listed
in the Output Window along with GC, Tm, and length.  What does that score
mean?  When I started using Gap4, I just used the first primer that was
chosen.  Now that I noticed the score values, I've been using primers with
the highest scores.  However, those primers have not been working.  Am I
doing something wrong?  Thanks.

Paul Shinn    
Sequencing Coordinator                                    ,___o
pshinn at neomorph.bio.upenn.edu                            _-\_<,
Arabidopsis thaliana Genome Center                      (*)/'(*)
(215) 573-7256

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