Hallo,
my name is Frank Vorhoelter. I'm a Ph.D. student, trying to sequence a
cosmid of Xanthomonas campestris DNA. By now, I have a couple of
sequence reads, which I try to assemble via pregap ang gap4. Some of
these reads are derived from common PCR templates. In the gap4 manual, I
saw hints suggesting gap4 might be able to use such information to
highlight relationships between templates and reads. However, I do not
know how to tell gap4 which read origins from which template. When I
enter reads through pregap, for every read a new template is created.
For any kind of help I'd be very thankful, as I am not a computing
expert.
Kind regards, and thanks in advance
Frank
--
Frank-Joerg Vorhoelter
Biologie VI (Genetik) Tel. ++49-(0)521-1062036
Universitaet Bielefeld Fax ++49-(0)521-1065626
Universitaetsstr. 25
D-33615 Bielefeld e-mail frank at genetik.uni-bielefeld.de
Germany
